96 Tests PN: B112469

Components:

45x Ab-conjugated beads (S5P4 - Human CCL28 Ab-bead). PN: B112469A. One vial containing 100 µL of anti-Human CCL28 conjugated to AimPlex Bead S5P4.

25x Biotin-detection Ab (Human CCL28 Biotin-dAb). PN: B112469B. One vial containing 100 µL of biotinylated anti-Human CCL28.

Lyophilized Standard Mix-Human Group 6, Panel A, 7-Plex PN: HG61007. One vial containing lyophilized recombinant 6Ckine, BRAK, BCA-1, CTACK, CCL28, ENA-78, and CXCL16.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits

Application: Optimal antibody pair and antigen standard for assaying human CCL28/SCYA28/MEC. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 6: Negligible

  • Sample volume: 15 µL/test

Description:

CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.] This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.  CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

References:

  1. Rodriguez et al. 2004. Differential gene expression by integrin 7+ and 7– memory T helper cells. BMC Immunol. 5: 13.

  2. Kunkel et al. 2003. CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells. J. Clin. Invest. 111: 1001-1010.

  3. John et al. 2005, Temporal production of CCL28 corresponds to eosinophil accumulation and airway hyperreactivity in allergic airway inflammation, Am. J. Pathol. 166: 345–353.

  4. Hieshima et al. 2003. CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. J. Immunol. 170: 1452-1461.

  5. Wilson and Butcher. CCL28 controls immunoglobulin (Ig)A plasma cell accumulation in the lactating mammary gland and IgA antibody transfer to the neonate. J Exp Med. 2004 Sep 20;200(6):805-9.

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