96 Tests PN: A111122

32 Tests PN: A111122T

Components:

45x Ab-conjugated beads (S4P9 - human IFNγ ab-bead).

  • 96 Tests PN: A111122A. One vial containing 100 µL of anti-human IFNγ conjugated to AimPlex Bead S4P9.

  • 32 Tests PN: A111122TA. One vial containing 35 µL of anti-human IFNγ conjugated to AimPlex Bead S4P9.

25x Biotin-detection Ab(human IFNγ Biotin-dAb).

  • 96 Tests PN: A111122B. One vial containing 100 µL of biotinylated anti-human IFNγ.

  • 32 Tests PN: A111122TB. One vial containing 35 µL of biotinylated anti-human IFNγ.

Lyophilized Standard Mix-Human Group 1 Panel A, 7-Plex.

  • 96 & 32 Tests PN: HG10007. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

Application: Optimal antibody pair and antigen standard for assaying human IFNγ.  Can be multiplexed with other analytes in Human Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (96 Tests PN: P100001, 32 Tests PN: P100001T ) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 3 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 2,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interferon-gamma (IFNg), also known as type II or immune interferon exerts a wide range of immune-regulatory activities and is considered to be the prototype pro-inflammatory cytokine.  IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemo-attraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, anti-proliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

References:

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.

  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.

  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.

  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.

  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.