96 Tests PN: B117338

Components:

45x Ab-conjugated beads (S5P7 - Human IgE Ab-bead). PN: B117338A. One vial containing    100 µL of anti-human IgE conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (Human IgE Biotin-dAb). PN: B117338B. One vial containing 100 µL of biotinylated anti-human IgE.

Lyophilized Standard Mix - Human IgE. PN: B117338S. One vial containing lyophilized IgE.

Application: Optimal antibody pair and antigen standard for assaying human Human IgE. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Note: Some specimens may contain high level of IgE. Pre-diluting normal serum and plasma samples 200 folds with the Sample Dilution Buffer (PN: P830100) is recommended for the IgE assay. Dilution factors should be optimized according to specific experimental conditions and sample types.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 50,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Immunoglobulin E (IgE) is an immunoglobulin (Ig) that has only been found in mammals and is synthesized by plasma cells. IgE's main function is immunity to parasites such as helminths e.g.Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE is utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. IgE also has an essential role in type I hypersensitivity, which manifests in various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergies, and specific types of chronic urticaria and atopic dermatitis. IgE has been shown to play a pivotal role in responses to allergens, such as: anaphylactic drugs, bee stings, and antigen preparations used in desensitization immunotherapy.

Although IgE is typically the least abundant isotype—blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 75% for the IgGs at 10 mg/ml, which are the isotypes responsible for most of the classical adaptive immune response—it is capable of triggering the most powerful inflammatory reactions.

References:

  1. Erb KJ (2007). "Helminths, allergic disorders and IgE-mediated immune responses: where do we stand?". Eur. J. Immunol. 37 (5): 1170–3. doi:10.1002/eji.200737314. PMID 17447233.

  2. Fitzsimmons CM, McBeath R, Joseph S, Jones FM, Walter K, Hoffmann KF, Kariuki HC, Mwatha JK, Kimani G, Kabatereine NB, Vennervald BJ, Ouma JH, Dunne DW (2007). "Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: Molecular structure and patterns of in vivo exposure". Int. Arch. Allergy Immunol. 142 (1): 40–50. doi:10.1159/000095997. PMID 17019080.

  3. Watanabe N, Bruschi F, Korenaga M (2005). "IgE: a question of protective immunity in Trichinella spiralis infection". Trends Parasitol. 21 (4): 175–8. doi:10.1016/j.pt.2005.02.010. PMID 15780839.

  4. Pfister K, Turner K, Currie A, Hall E, Jarrett EE (1983). "IgE production in rat fascioliasis". Parasite Immunol. 5 (6): 587–93. doi:10.1111/j.1365-3024.1983.tb00775.x. PMID 6657297.