96 Tests PN: B111419

32 Tests PN: A111419T

Components:

45x Ab-conjugated beads (S5P7 - human IL-33 Ab-bead).

  • 96 Tests PN: B111419A. One vial containing 100 µL of anti-human IL-33 conjugated to AimPlex Bead S5P7.

  • 32 Tests PN: A111419TA. One vial containing 35 µL of anti-human IL-33 conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (human IL-33 Biotin-dAb).

  • 96 Tests PN: B111419B. One vial containing 100 µL of biotinylated anti-human IL-33.

  • 32 Tests PN: A111419TB. One vial containing 35 µL of biotinylated anti-human IL-33.

Lyophilized Standard Mix-Human Group 2 Panel B, 10-Plex.

  • 96 & 32 Tests PN: HG20010. One vial containing lyophilized recombinant human IL-17F, IL-20, IL-21, IL-28A, IL-29, IL-33, M-CSF, MPO, TSLP, and VEGF-A. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

Application: Optimal antibody pair and antigen standard for assaying human IL-33.  Can be multiplexed with other analytes in Human Group 2.  To be used in conjunction with the AimPlex NR Basic Kit (96 Tests PN: P100001, 32 Tests PN: P100001T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin 33 (Accession O95760) is a member of the Interleukin-1 cytokine family. Like Il-1α, it is a dual function protein, as it is a nuclear protein but may also be released into the extracellular space. IL-33 is not secreted through conventional pathways and aids in mediating parasitic and bacterial infections. IL-33 can also work in conjunction with other inflammatory cytokines to promote responses by NK cells, Th1 and CD8 T cells. It is implicated in many inflammatory diseases like rheumatoid arthritis, asthma, and atherosclerosis.

References:

  1. Palmer G, Gabay C. Interleukin-33 biology with potential insights into human diseases. Nature Rev Rheum. 2011; 7(6): 321-329. Doi: 10.1038/nrrheum.2011.53.

  2. Molofsky AB, Savage AK, Locksley RM. Interleukin-33 in Tissue Homeostasis, Injury, and Inflammation. Immunity. 2015; 42(6): 1005-1019. Doi: 10.1016/j.immuni.2015.06.006.