96 Tests PN: B112494

Components:

45x Ab-conjugated beads (S4P8- Human MCP-2 Ab-bead). PN: B112494A. One vial containing 100 µL of anti-Human MCP-2 conjugated to AimPlex Bead S4P8.

25x Biotin-detection Ab (Human MCP-2 Biotin-dAb). PN: B112494B. One vial containing 100 µL of biotinylated anti-Human MCP-2.

Lyophilized Standard Mix-Human Group 7, Panel A, 8-Plex. PN: HG71008. One vial containing lyophilized recombinant Eotaxin-3, GCP-2, GROα, HCC-1, HCC-4, I-309, MCP-2, and MPIF-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human CCL8/SCYA8/MCP-2. Can be multiplexed with other analytes in Human Group 7.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 7: Negligible

  • Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2) and small inducible cytokine A8 (SYCA8), is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans.  MCP-2 is chemotactic for and activates a many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to CCR1, CCR2B and CCR5 chemokine receptors. MCP-2 is a potent inhibitor of HIV-1 by virtue of its high-affinity binding to the receptor CCR5, one of the major co-receptors for HIV-1.

References:

  1. PDB: 1ESR; Blaszczyk J, Coillie EV, Proost P, Damme JV, Opdenakker G, Bujacz GD, Wang JM, Ji X (November 2000). "Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors". Biochemistry 39 (46): 14075–81. doi:10.1021/bi0009340. PMID 11087354.

  2. Van Coillie E, Fiten P, Nomiyama H, Sakaki Y, Miura R, Yoshie O, Van Damme J, Opdenakker G (March 1997). "The human MCP-2 gene (SCYA8): cloning, sequence analysis, tissue expression, and assignment to the CC chemokine gene contig on chromosome 17q11.2". Genomics 40 (2): 323–31. doi:10.1006/geno.1996.4594. PMID 9119400.

  3. Van Damme J, Proost P, Lenaerts JP, Opdenakker G (July 1992). "Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family". J. Exp. Med. 176 (1): 59–65. doi:10.1084/jem.176.1.59. PMC 2119277. PMID 1613466.