96 Tests PN: B111431

Components:

45x Ab-conjugated beads (S5P5 - human MIF Ab-bead). PN: B111431A. One vial containing 100 µL of anti-human MIF conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (human MIF Biotin-dAb). PN: B111431B. One vial containing 100 µL of biotinylated anti-human MIF.

Lyophilized Standard Mix-Human Group 4 Panel B, 10-Plex. PN: HG40010. One vial containing lyophilized recombinant human GASP-1, IFNβ, ANGPT-1, Insulin, Leptin, MIF, Resistin, Survivin, TGFα, and TPO.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human MIF.  Can be multiplexed with other analytes in Human Group 4.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 4: Negligible

  • Sample volume: 15 µL/test

Description:

Macrophage migration inhibitory factor (MIF or MMIF), also known as glycosylation-inhibiting factor (GIF), L-Dopachrome Isomerase, or Phenylpyruvate Tautomerase is a protein that is an important regulator of innate immunity. Bacterial antigens stimulate white blood cells to release MIF into the blood stream.  The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. Hence, MIF is classified as an inflammatory cytokine. Furthermore glucocorticoids also stimulate white blood cells to release MIF and hence MIF partially counteracts the inhibitory effects that glucocorticoids have on the immune system. Trauma activates the anterior pituitary gland to release MIF.  MIF is an inflammatory mediator associated with rheumatoid arthritis severity. MIF protein levels are increased in the plasma of people with schizophrenia, including people in the early stages of the illness, but the clinical significance of this has not been determined. It has also been shown to induce changes in the heart during sepsis.  Evidence suggests that there is a correlation between MIF production and metastatic potential in colorectal cancer.

References:

  1. Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR (October 1989). "Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor". Proc. Natl. Acad. Sci. U.S.A. 86 (19): 7522–6. doi:10.1073/pnas.86.19.7522. PMC 298097. PMID 2552447.

  2. Kozak CA, Adamson MC, Buckler CE, Segovia L, Paralkar V, Wistow G (June 1995). "Genomic cloning of mouse MIF (macrophage inhibitory factor) and genetic mapping of the human and mouse expressed gene and nine mouse pseudogenes". Genomics 27 (3): 405–11. doi:10.1006/geno.1995.1070. PMID 7558020.

  3. Calandra T, Roger T (October 2003). "Macrophage migration inhibitory factor: a regulator of innate immunity". Nat. Rev. Immunol. 3 (10): 791–800. doi:10.1038/nri1200. PMID 14502271.

  4. Leng L, Bucala R (February 2006). "Insight into the biology of macrophage migration inhibitory factor (MIF) revealed by the cloning of its cell surface receptor". Cell Res. 16 (2): 162–8. doi:10.1038/sj.cr.7310022. PMID 16474429.