96 Tests PN: A112242

Components:

45x Ab-conjugated beads (S5P7 - human MIP-1β Ab-bead). PN: A112242A. One vial containing 100 µL of anti-human MIP-1β conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (human MIP-1β Biotin-dAb). PN: A112242B. One vial containing 100 µL of biotinylated anti-human MIP-1β.

Lyophilized Standard Mix-Human Group 3 Panel A, 8-Plex. PN: HG30008. One vial containing lyophilized recombinant human Fractalkine, LIF, TNFβ, VEGF-D, I-TAC, MIP-1α, MIP-1β, and TIM-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human MIP-1β.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

Macrophage inflammatory protein-1β (Accession P13236) is a CC chemokine. Also known as CCL4, it is produced by neutrophils, monocytes, T & B cells, and endothelial and epithelial cells. MIP-1β acts as a chemoattractant for various types of immune cells and in conjunction with RANTES and MIP-1α, is a major HIV-suppressive factor. Its receptor, CCR5 stimulates production of IL-2 and IFN-γ, both of which have been observed in patients with sarcoidosis. Concentration of MIP-1β within the body increases with age, while it also maintains an inverse relationship to the concentration of MicroRNA-125b. Elevated levels of MIP-1β has been correlated to patients with Kawasaki disease.

References:

  1. Capelli A, di Stefano A, Lusuardi M, Gnemmi I, Donner CF. Increased Macrophage Inflammatory Protein-1 α and Macrophage Inflammatory Protein-1 β in Bronchoalveolar Lavage Fluid of Patients Affected by Different Stages of Pulmonary Sarcoidosis. AJRCCM. 2002; 165(2). Doi: 10.1164/ajrccm.165.2.2106084.

  2. Cheng NL, Chen X, Kim J, Shi AH, Nguyen C, Wersto R, Weng NP. MicroRNA-125b modulates inflammatory chemokine CCL4 expression in immune cells and its reduction causes CCL4 increase with age. Aging Cell. 2015; 14(2): 200-8. Doi: 10.1111/acei.12294.

  3. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P. Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T Cells. Science. 1995; 270(5243): 1811-5. Doi: 10.1126/science.270.5243.1811.

  4. Wong M, Sliverman ED, Fish EN. Evidence for RANTES, monocyte chemotactic protein-1, and macrophage inflammatory protein-1 beta expression in Kawasaki disease. J Rheum. 1997; 24(6):1179-1185. PMID: 9195529.