96 Tests PN: B111194

Components:

45x Ab-conjugated beads (S4P3 - human PDGF-AB Ab-bead). PN: B111194A. One vial containing 100 µL of anti-human PDGF-AB conjugated to AimPlex Bead S4P3.

25x Biotin-detection Ab (human PDGF-AB Biotin-dAb). PN: B111194B. One vial containing 100 µL of biotinylated anti-human PDGF-AB.

Lyophilized Standard Mix-Human Group 4 Panel A. PN: HG4009A. One vial containing lyophilized recombinant human BDNF, beta-NGF, EGF, FGF basic, OPG, OPN, PDGF-AB and VEGF-C.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human PDGF-AB.  Can be multiplexed with other analytes in Human Group 4.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Important: Sample types containing low levels of platelets such as platelet-poor plasma are recommended.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, platelet-poor plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 4: Negligible

  • Sample volume: 15 µL/test

Description:

Platelet-derived growth factor-AB (PDGF-AB) is one of the five dimeric proteins belonging to the PDGF family. Other members are homodimers PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGFs are produced by platelets, stored in platelet alpha-granules and are released upon platelet activation. PDGF proteins are generally considered as potent mitogens for connective tissue cells, and can also be chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. PDGF-AB is reported to support angiogenesis in the heart. PDGF-AB is able to bind to both PDGFR alpha and PDGFR beta.

References:

  1. Nister M, Hammacher A, Mellstrom K, Siegbahn A, Ronnstrand L, Westermark B and Heldin CH. 1988. Cell. 52(6): 791-799.

  2. Fredriksson L, Li H and Eriksson U. 2004. Cytokine Growth Factor Rev. 15(4): 197-204.

  3. Xaymardan M, Tang L, Zagreda L, Pallante B, Zheng J, Chazen JL, Chin A, Duignan I, Nahirney P, Rafii S, Mikawa T, Edelberg JM. 2004. Circ Res. 94(5): E39-45.

  4. Li X and Eriksson U. 2003. Cytokine Growth Factor Rev. 14(2): 91-98