96 Tests PN: B211221
Components:
45x Ab-conjugated beads (S5P4 - Mouse BTC Ab-bead). PN: B211221A. One vial containing 100 µL of anti-mouse BTC conjugated to AimPlex Bead S5P4.
25x Biotin-detection Ab (Mouse BTC Biotin-dAb). PN: B211221B. One vial containing 100 µL of biotinylated anti-mouse BTC.
Lyophilized Standard Mix - Mouse Group 3 Panel A, 8-Plex. PN: MG3008A. One vial containing lyophilized recombinant mouse BTC, IL-1RA, IL-9, IL-23p19, IL-25, Leptin, LIF and VEGF. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human Mouse BTC/Betacellulin. Can be multiplexed with other analytes in Mouse Group 3. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 2 pgmL
Quantitation range:
LLOQ: < 5 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Mouse Group 3: Negligible
Sample volume: 15 µL/test
Description:
Betacellulin (Accession Q05928) is a growth factor of the EGF family. It’s primary area of function is in the pancreas. BTC promotes differentiation and growth of pancreatic beta cells. It also has effects on the exocrine, endocrine, and ductal differentiation. BTC inhibits expression of amylase and glucagon but increases expression of insulin and ghrelin in in vitro mouse pancreases. Unlike other epidermal growth factors, BTC demonstrates some pro-apoptotic action in the pancreas
References:
Li L, Seno M, Yamada H, Kojima I. Promotion of β-cell regeneration by Betacellulin in ninety percent-pancreatectomized rats. Endocrinology. 2001; 142(12): 5379-5385. Doi: 10.1210/endo.142.12.8520.
Thowfeequ S, Ralphs KL, Yu WY, Slack JMW, Tosh D. Betacellulin inhibits amylase and glucagon production and promotes beta cells differentiation in mouse embryonic pancreas. Diabetologia. 2007; 50(8): 1688-1697. Doi: 10.1007/s00125-007-0724-y.