96 Tests PN: B311165

Components:

45x Ab-conjugated beads (S5P9 - Rat IL-1β Ab-bead). PN: B311165A. One vial containing 100 µL of anti-rat IL-1β conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Rat IL-1β Biotin-dAb). PN: B311165B. One vial containing 100 µL of biotinylated anti-rat IL-1β.

Lyophilized Standard Mix - Rat Group 1 Panel B, 12-Plex. PN: RG10012. One vial containing lyophilized recombinant rat Eotaxin, G-CSF, GM-CSF, IL-1α, IL-1β, IL-5, IL-12p70, IL-13, KC, IP-10, MIP-1α, MIP-2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Rat IL-1β/IL-1F2. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Rat Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin-1 beta (Accession P43527) is a member of Interleukin 1 subfamily, known for their association with acute and chronic inflammation. IL-1β secretion is different from most cytokines as it may be secreted through Golgi or ER independent processes. IL-1β acts as the key mediator in inflammatory responses and cellular activities such as proliferation, differentiation, and apoptosis within the innate immune system. Over expression of IL-1 has been associated with increased risk for Alzheimer’s disease, arthritis, and other degenerative brain conditions.

References:

  1. Griffin WS, Mrak RE. Interleukin-1 in the genesis and progression of and risk for development of neuronal degeneration in Alzheimer’s disease. J Leukoc Biol. 2002; 72(2): 233-238. PMID: 12149413.

  2. Monteleone M, Stow JL, Schroder K. Mechanisms of unconventional secretion of IL-1 family cytokines. Cytokine. 2015; 74 (2): 213-218. Doi: 10.1016/j.cyto.2015.03.022.