• PN: A111149T

45x Ab-conjugated beads (S4P5 - human IL-17F Ab-bead). PN: A111149TA. One vial containing 35 µL of anti-human IL-17F conjugated to AimPlex Bead S4P5.

25x Biotin-detection Ab (human IL-17F Biotin-dAb). PN: A111149TB. One vial containing 35 µL of biotinylated anti-human IL-17F.

Lyophilized Standard Mix-Human Group 2 Panel B, 10-Plex. PN: HG20010. One vial containing lyophilized recombinant human IL-17F, IL-20, IL-21, IL-28A, IL-29, IL-33, M-CSF, MPO, TSLP, and VEGF-A. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-17F.  Can be multiplexed with other analytes in Human Group 2.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 2 pg/mL

Quantitation range:

LLOQ: < 5 pg/mL

ULOQ: > 2,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 2: Negligible

Sample volume: 15 µL/test

Description:

Interleukin 17F (IL-17F) is a cytokine expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. IL17F has also been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Diseases associated with IL17F include candidiasis, familial, 6, autosomal dominant and chronic mucocutaneous candidiasis. Among its related pathways are IL-17 Family Signaling Pathways and IL-17 Family Signaling Pathways.

References:

1.      Association analysis of the interleukin 17 genes IL17A and IL17F as potential osteoarthritis susceptibility loci. Southam, L., Heath, O., Chapman, K., Loughlin, J. Ann. Rheum. Dis. (2006) [Pubmed]

2.      Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony-stimulating factor in bronchial epithelium: implications for airway inflammation in cystic fibrosis. McAllister, F., Henry, A., Kreindler, J.L., Dubin, P.J., Ulrich, L., Steele, C., Finder, J.D., Pilewski, J.M., Carreno, B.M., Goldman, S.J., Pirhonen, J., Kolls, J.K. J. Immunol. (2005) [Pubmed]

3.      IL-17 cytokine family. Kawaguchi, M., Adachi, M., Oda, N., Kokubu, F., Huang, S.K. J. Allergy Clin. Immunol. (2004) [Pubmed]

4.      Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response. Oda, N., Canelos, P.B., Essayan, D.M., Plunkett, B.A., Myers, A.C., Huang, S.K. Am. J. Respir. Crit. Care Med. (2005) [Pubmed]