PN: A111155T

COMPONENTS

45x Ab-conjugated beads (S4P7 - human IL-2 ab-bead). PN: A111155TA. One vial containing 35 µL of anti-human IL-2 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (human IL-2 Biotin-dAb). PN: A111155TB. One vial containing 35 µL of biotinylated anti-human IL-2.

Lyophilized Standard Mix-Human Group 1 Panel A, 7-Plex. PN: HG10007. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-2.  Can be multiplexed with other analytes in Human Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 2,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 1: Negligible

Sample volume: 15 µL/test

Description:

Interleukin 2 (Accession P60568) is a member of the interleukin cytokine family secreted primarily by activated CD4+ and CD8+ T-cells, NK cells, and dendritic cells. IL-2 participates in immune responses but also in delivering signals for thymic development, which in turn promotes homeostasis of T-cells. IL-2 promotes T-cell differentiation into effector, regulatory, or memory T-cells. Elevated levels of IL-2 may be correlated with blood hypereosinophilia, hemophagocytic lymphohistiocytosis, and a range of cardiopulmonary toxicities such as tachycardia and dyspnea.

References:

  1. Hayden A, Lin M, Park S, Pudek M, Schneider M, Jordan MB, Mattman A, Chen LYC. Soluble interleukn-2 receptor is a sensitive diagnostic test in adult HLH. Blood Advances. 2017; 1(26): 2529-2534. Doi: 10.1182/bloodadvances.2017012310

  2. Letourneau S, van Leeuwen EMM, Krieg C, Martin C, Pantaleo G, Sprent J, Surh CD, Boyman O. Il-2/anti-IL-2 antibody complexes show strong biological activity by avoiding interaction with IL-2 receptor α subunit CD25. PNAS. 2010; 107(5): 2171-2176. Doi:10.1073/pnas.0909384107.

  3. Liao W, Lin JX. Leonard WJ. IL-2 family cytokines: new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation. Curr. Opin. Immunology. 2011; 23 (5): 598-604. Doi:10.1016/j.coi.2011.08.003.

  4. Malek TR, Castro I. Interleukin-2 Receptor Signaling: At the Interface between Tolerance and Immunity. Immunity. 2010; 33 (2): 153-165. Doi: 10.1016/j.immuni.2010.08.004.

  5. Prin L, Plumas J, Gruart V, Loiseau S, Aldebert D, Ameisen JC, Vermersch A, Fenaux P, Bletry O, Capron M. Elevated Serum Levels of Soluble Interleukin-2 Receptor: A Marker of Disease Activity in the Hypereosinophilic Syndrome. Blood Journal. 1991; 78: 2626-2632. ISSN: 1528-0020.