PN: C195516T

COMPONENTS:

1x Ab-conjugated beads (premixed). PN: C195516TA. One bottle containing 1.5 mL of the following AimPlex antibody-bead conjugates:

S4P2 - anti-human MIP-1α

S4P3 - anti-human TNFα

S4P6 - anti-human sCD137

S4P7 - anti-human IL-2

S4P8 - anti-human MIP-1β

S4P9 - anti-human IFNγ

S4P10 - anti-human Granzyme B

S4P11 - anti-human IL-15

S5P2 - anti-human sFasL

S5P3 - anti-human GM-CSF

S5P4 - anti-human IL-4

S5P5 - anti-human IL-5

S5P6 - anti-human IL-6

S5P7 - anti-human RANTES

S5P10 - anti-human IL-10

S5P12 - anti-human IL-13

2x Biotin-detection Ab (premixed). PN: C195516TB. One vial containing 0.45 mL of the following biotinylated antibodies:

Biotin - anti-human MIP-1α

Biotin - anti-human TNFα

Biotin - anti-human sCD137

Biotin - anti-human IL-2

Biotin - anti-human MIP-1β

Biotin - anti -human IFNγ

Biotin - anti-human Granzyme B

Biotin - anti-human IL-15

Biotin - anti-human sFasL

Biotin - anti-human GM-CSF

Biotin - anti-human IL-4

Biotin - anti-human IL-5

Biotin - anti-human IL-6

Biotin - anti-human RANTES

Biotin - anti-human IL-10

Biotin - anti-human IL-13

2x NR dAb Diluent. PN: 07NDX21T. One vial containing 0.45 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Human CD8+ T Cell Related Cytokines, 16-plex. PN: HG19516. One vial containing lyophilized recombinant GM-CSF, Granzyme B, IFNγ, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15, MIP-1α, MIP-1β, RANTES, sCD137, sFasL, TNFα.  

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels - 32 Tests: 

      1x Streptavidin-PE (SAPE). PN: 07SP0011T. One bottle containing 1 mL of 1x SAPE.

      10x Reading Buffer. PN: 07RB1T. One bottle containing 1.5 mL of 10x buffer.

      10x Wash Buffer. PN: 07WB1T. One vial containing 5 mL of 10x buffer.

      PCR 8-tube Strip. PN: 07P8T1. 2 strips.

      Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

      Plate Seals. PN: 07PSR1. 3 sealers.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying human CD8+ T Cell Related Cytokines, 16-plex.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 32 Tests (PN: U100005T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.   

Assay Specifications: 

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): GM-CSF, < 5 pg/mL; Granzyme B, < 10 pg/mLIFNγ, < 3 pg/mL; IL-2, < 2 pg/mL; IL-4, < 1 pg/mL; IL-5, < 2 pg/mL; IL-6, < 5 pg/mL; IL-10, < 2 pg/mL; IL-13, < 5 pg/mL; IL-15, < 5 pg/mL; MIP-1α, < 2 pg/mL; MIP-1β, < 5 pg/mL; RANTES, < 5 pg/mL; sCD137, < 5 pg/mL; sFasL, < 2 pg/mL; TNFα, < 1 pg/mL.

LLOQs: GM-CSF, < 10 pg/mL; Granzyme B, < 20 pg/mLIFNγ, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4, < 3 pg/mL; IL-5, < 5 pg/mL; IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-13, < 10 pg/mL; IL-15, < 10 pg/mL; MIP-1α, < 5 pg/mL; MIP-1β, < 10 pg/mL; RANTES, < 10 pg/mL; sCD137, < 10 pg/mL; sFasL, < 5 pg/mL; TNFα, < 2 pg/mL.

ULOQs: GM-CSF, > 5,000 pg/mL; Granzyme B, > 5,000 pg/mL; IFNγ, > 2,000 pg/mL; IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mL; IL-5, > 2,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 5,000 pg/mL; IL-13, > 5,000 pg/mL; IL-15, > 5,000 pg/mL; MIP-1α, > 2,000 pg/mL; MIP-1β, > 5,000 pg/mL; RANTES, > 5,000 pg/mL; sCD137, > 5,000 pg/mL; sFasL, > 2,000 pg/mL; TNFα, > 1,000 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

For Research Use Only.  Not for use in diagnostic procedures.