PN: C192216

COMPONENTS:

1x Ab-conjugated beads (premixed). PN: C192216A. One bottle containing 5 mL of the following AimPlex antibody-bead conjugates:

S4P2 - anti-human MIP-1α

S4P3 - anti-human TNFα

S4P4 - anti-human IP-10

S4P6 - anti-human IL-1β

S4P7 - anti-human IL-27

S4P9 - anti-human IFNγ

S4P10 - anti-human IL-8

S4P11 - anti-human IL-31

S4P12 - anti-human IL-12p70

S5P1 - anti-human MCP-1

S5P2 - anti-human IL-1α

S5P6 - anti-human IL-6

S5P7 - anti-human IL-33

S5P9 - anti-human IL-17A

S5P10 - anti-human IL-10

S5P12 - anti-human IL-13

2x Biotin-detection Ab (premixed). PN: C192216B. One vial containing 1.5 mL of the following biotinylated antibodies.

Biotin - anti-human MIP-1α

Biotin - anti-human TNFα

Biotin - anti-human IP-10

Biotin - anti-human IL-1β

Biotin - anti-human IL-27

Biotin - anti -human IFNγ

Biotin - anti-human IL-8

Biotin - anti-human IL-31

Biotin - anti-human IL-12p70

Biotin - anti-human MCP-1

Biotin - anti-human IL-1α

Biotin - anti-human IL-6

Biotin - anti-human IL-33

Biotin - anti-human IL-17A

Biotin - anti-human IL-10

Biotin - anti-human IL-13

2x NR dAb Diluent. PN: 07NDX21. One vial containing 1.5 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Human Inflammation 16-Plex. PN: HG19216. Vials A, B and C containing lyophilized recombinant human IFNγ, IL-1α, IL-1βa, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, TNFα. 

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels: 

      1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 3 mL of 1x SAPE.

      10x Reading Buffer. PN: 07RB1. One bottle containing 5 mL of 10x buffer.

      10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.

      PCR 8-tube Strip. PN: 07P8T1. 2 strips.

      Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

      Plate Seals. PN: 07PSR1. 6 sealers.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying Inflammation 16-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.   

Assay Specifications: 

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): IFNγ, < 3 pg/mL; IL-1α, < 2 pg/mLIL-, < 5 pg/mLIL-6, < 5 pg/mL; IL-8, < 1 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-13, < 5 pg/mL; IL-17A, < 3 pg/mL; IL-27, < 5 pg/mL; IL-31, < 5 pg/mL; IL-33, < 5 pg/mL; IP-10, < 3 pg/mL; MCP-1, < 5 pg/mL; MIP-1α, < 2 pg/mLTNFα, < 1 pg/mL.

LLOQs: IFNγ, < 10 pg/mL; IL-1α, < 5 pg/mLIL-< 10 pg/mLIL-6, < 10 pg/mL; IL-8, < 1 pg/mLIL-10, < 5 pg/mL;  IL-12p70, < 10 pg/mLIL-13, < 10 pg/mL; IL-17A, < 10 pg/mL; IL-27, < 10 pg/mL; IL-31, < 10 pg/mLIL-33, < 10 pg/mL; IP-10, < 10 pg/mL; MCP-1, < 5 pg/mL; MIP-1α, < 5 pg/mLTNFα, < 2 pg/mL.

ULOQs: IFNγ, > 2,000 pg/mL; IL-1α, > 2,000 pg/mL; IL-, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-8, > 1,000 pg/mL;  IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-13, > 5,000 pg/mLIL-17A, > 1,000 pg/mLIL-27, > 5,000 pg/mL; IL-31, > 5,000 pg/mLIL-33, > 5,000 pg/mLIP-10, > 2,000 pg/mL; MCP-1, > 5,000 pg/mL; MIP-1α, > 2,000 pg/mL; TNFα, > 1,000 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

For Research Use Only.  Not for use in diagnostic procedures.