PN: C191118K

COMPONENTS:

1x Ab-conjugated beads (premixed). PN: C191118A. One bottle containing 5 mL of the following AimPlex antibody-bead conjugates:

S4P2 - anti-human TNFβ

S4P3 - anti-human TNFα

S4P6 - anti-human IL-1β

S4P7 - anti-human IL-2

S4P8 - anti-human IL-17F

S4P9 - anti-human IFNγ

S4P10 - anti-human IL-8

S4P11 - anti-human IL-9

S4P12 - anti-human IL-12p70

S5P2 - anti-human IL-22

S5P3 - anti-human GM-CSF

S5P4 - anti-human IL-4

S5P5 - anti-human IL-5

S5P6 - anti-human IL-6

S5P7 - anti-human IL-33

S5P9 - anti-human IL-17A

S5P10 - anti-human IL-10

S5P11 - anti-human TSLP

2x Biotin-detection Ab (premixed). PN: C191118B. One vial containing 1.5 mL of the following biotinylated antibodies.

Biotin - anti-human TNFβ

Biotin - anti-human TNFα

Biotin - anti-human IL-1β

Biotin - anti-human IL-2

Biotin - anti-human IL-17F

Biotin - anti -human IFNγ

Biotin - anti-human IL-8

Biotin - anti-human IL-9

Biotin - anti-human IL-12p70

Biotin - anti-human IL-22

Biotin - anti-human GM-CSF

Biotin - anti-human IL-4

Biotin - anti-human IL-5

Biotin - anti-human IL-6

Biotin - anti-human IL-33

Biotin - anti-human IL-17A

Biotin - anti-human IL-10

Biotin - anti-human TSLP

2x NR dAb Diluent. PN: 07NDX21. One vial containing 1.5 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Human TH1/Th2/Th17 18-plex. PN: HG19118. Vials A, B and C containing lyophilized recombinant human GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-17A, IL-17F, IL-22, IL-33, TNFα, TNFβ and TSLP. 

Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying Th1/Th2/Th17 18-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.   

Assay Specifications: 

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): GM-CSF, < 5 pg/mLIFNγ, < 3 pg/mLIL-, < 5 pg/mLIL-2, < 3 pg/mLIL-4, < 1 pg/mLIL-5, < 2 pg/mLIL-6, < 5 pg/mL; IL-8, < 1 pg/mLIL-9, < 5 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-17A, < 3 pg/mL; IL-17F, < 2 pg/mLIL-22, < 3 pg/mLIL-33, < 5 pg/mLTNFα, < 1 pg/mLTNFβ, < 5 pg/mLTSLP, < 2 pg/mL.

LLOQs: GM-CSF, < 10 pg/mLIFNγ, < 10 pg/mLIL-< 10 pg/mLIL-2, < 10 pg/mLIL-4, < 3 pg/mLIL-5, < 5 pg/mL; IL-6, < 10 pg/mL; IL-8, < 1 pg/mLIL-9, < 10 pg/mL; IL-10, < 5 pg/mL;  IL-12p70, < 10 pg/mL; IL-17A, < 10 pg/mLIL-17F, < 5 pg/mLIL-22, < 10 pg/mLIL-33, < 10 pg/mLTNFα, < 2 pg/mLTNFβ, < 10 pg/mLTSLP, < 5 pg/mL.

ULOQs: GM-CSF,  > 5,000 pg/mL; IFNγ, > 2,000 pg/mL; IL-, > 5,000 pg/mL;  IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mLIL-5, > 2,000 pg/mL; IL-6, > 5,000 pg/mL; IL-8, > 1,000 pg/mL; IL-9, > 5,000 pg/mL; IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL;  IL-17A, > 1,000 pg/mLIL-17F, > 2,000 pg/mLIL-22, > 2,000 pg/mLIL-33, > 5,000 pg/mL; TNFα, > 1,000 pg/mLTNFβ,  > 5,000 pg/mL; TSLP, > 2,000 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

For Research Use Only.  Not for use in diagnostic procedures.