PN: C281114T

COMPONENTS:

1x Ab-conjugated beads (premixed). PN:  C281114TA. One bottle containing 1.5 mL of the following AimPlex antibody-bead conjugates:

S4P2 - anti-mouse IL-2

S4P4 - anti-mouse TNFα

S4P6 - anti-mouse IL-1α

S4P7 - anti-mouse IL-12p70

S4P8 - anti-mouse IFNγ

S4P9 - anti-mouse IL-22

S4P10 - anti-mouse IL-13

S4P12 - anti-mouse IL-17A

S5P2 - anti-mouse IL-4

S5P4 - anti-mouse IL-1β

S5P5 - anti-mouse IL-6

S5P7 - anti -mouse IL-5  

S5P9 - anti-mouse IL-10

S5P10 - anti-mouse IL-21

2x Biotin-detection Ab (premixed). PN: C281114TB. One vial containing 0.45 mL of the following biotinylated antibodies.

Biotin - anti-mouse IL-2

Biotin - anti-mouse TNFα

Biotin - anti-mouse IL-1α

Biotin - anti-mouse IL-12p70

Biotin - anti-mouse IFNγ

Biotin - anti-mouse IL-22

Biotin - anti-mouse IL-13

Biotin - anti-mouse IL-17A

Biotin - anti-mouse IL-4

Biotin - anti-mouse IL-1β

Biotin - anti-mouse IL-6

Biotin - anti - mouse IL-5  

Biotin - anti-mouse IL-10

Biotin - anti-mouse IL-21

2x Mouse/Rat dAb Diluent. PN: 07MRDX21T. One vial containing 0.45 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Mouse TH1/Th2/Th17 14-Plex. PN: MG28014. One vial containing lyophilized recombinant IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22 and TNFα.

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels - 32 Tests: 

      1x Streptavidin-PE (SAPE). PN: 07SP0011T. One bottle containing 1 mL of 1x SAPE.

      10x Reading Buffer. PN: 07RB1T. One bottle containing 1.5 mL of 10x buffer.

      10x Wash Buffer. PN: 07WB1T. One vial containing 5 mL of 10x buffer.

      PCR 8-tube Strip. PN: 07P8T1. 2 strips.

      Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

      Plate Seals. PN: 07PSR1. 3 sealers.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying the mouse Th1/Th2/Th17 14-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 21 Tests (PN: U100005T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure. 

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): IFNγ, < 0.5 pg/mL; IL-1α, < 2 pg/mL; IL-1β, < 2 pg/mL; IL-2, < 2 pg/mL; IL-4, < 0.5 pg/mL; IL-5, < 5 pg/mL; IL-6, < 3 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 1 pg/mL; IL-13, < 5 pg/mL; IL-17A, <1.5 pg/mL; IL-21, < 5 pg/mL; IL-22, < 5 pg/mL; TNFα, < 0.5 pg/mL.

LLOQs: IFNγ, < 2 pg/mL; IL-1α, < 10 pg/mL; IL-1β, < 5 pg/mL; IL-2, < 5 pg/mL; IL-4, < 2 pg/mL; IL-5, < 10 pg/mL; IL-6, < 10 pg/mL;  IL-10, < 5 pg/mL; IL-12p70, < 2 pg/mL; IL-13, < 10 pg/mL; IL-17A, < 5 pg/mL; IL-21, < 10 pg/mL; IL-22, < 10 pg/mL; TNFα, < 2 pg/mL.

ULOQs: IFNγ, > 5,000 pg/mL; IL-1α, > 5,000 pg/mL;  IL-1β, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-5, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-13, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; IL-21, > 5,000 pg/mL; IL-22, > 5,000 pg/mL; TNFα, > 1,500 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

 For Research Use Only.  Not for use in diagnostic procedures.