PN: C281116K

COMPONENTS:

1x Ab-conjugated beads (premixed). PN:  C281116A. One bottle containing 5 mL of the following AimPlex antibody-bead conjugates:

S4P2 - anti-mouse IL-2

S4P3 - anti-mouse IL-23p19

S4P4 - anti-mouse TNFα

S4P6 - anti-mouse IL-1α

S4P7 - anti-mouse IL-12p70

S4P8 - anti-mouse IFNγ

S4P10 - anti-mouse IL-13

S4P11 - anti-mouse GM-CSF

S4P12 - anti-mouse IL-17A

S5P2 - anti-mouse IL-4

S5P3 - anti-mouse KC

S5P4 - anti-mouse IL-1β

S5P5 - anti-mouse IL-6

S5P7 - anti -mouse IL-5  

S5P9 - anti-mouse IL-10

S5P10 - anti-mouse IL-21

2x Biotin-detection Ab (premixed). PN: C281116B. One vial containing 1.5 mL of the following biotinylated antibodies.

Biotin - anti-mouse IL-2

Biotin - anti-mouse IL-23p19

Biotin - anti-mouse TNFα

Biotin - anti-mouse IL-1α

Biotin - anti-mouse IL-12p70

Biotin - anti-mouse IFNγ

Biotin - anti-mouse IL-13

Biotin - anti-mouse GM-CSF

Biotin - anti-mouse IL-17A

Biotin - anti-mouse IL-4

Biotin - anti-mouse KC

Biotin - anti-mouse IL-1β

Biotin - anti-mouse IL-6

Biotin - anti - mouse IL-5  

Biotin - anti-mouse IL-10

Biotin - anti-mouse IL-21

2x Mouse/Rat dAb Diluent. PN: 07MRDX21. One vial containing 1.5 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Mouse TH1/Th2/Th17 16-Plex. PN: MG28016. One vial containing lyophilized recombinant GM-CSF, IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-23p19, KC, TNFα. 

Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying the mouse Th1/Th2/Th17 16-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure. 

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): GM-CSF, < 5 pg/mL; IFNγ, < 0.5 pg/mL; IL-1α, < 2 pg/mL; IL-1β, < 2 pg/mL; IL-2, < 2 pg/mL; IL-4, < 0.5 pg/mL; IL-5, < 5 pg/mL; IL-6, < 3 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 1 pg/mL; IL-13, < 5 pg/mL; IL-17A, <1.5 pg/mL; IL-21, < 5 pg/mL; IL-23p19, < 5 pg/mL; KC, < 1 pg/mL; TNFα, < 0.5 pg/mL.

LLOQs: GM-CSF, < 10 pg/mL; IFNγ, < 2 pg/mL; IL-1α, < 10 pg/mL; IL-1β, < 5 pg/mL; IL-2, < 5 pg/mL; IL-4, < 2 pg/mL; IL-5, < 10 pg/mL; IL-6, < 10 pg/mL;  IL-10, < 5 pg/mL; IL-12p70, < 2 pg/mL; IL-13, < 10 pg/mL; IL-17A, < 5 pg/mL; IL-21, < 10 pg/mL; IL-23p19, < 10 pg/mL; KC, < 2 pg/mL; TNFα, < 2 pg/mL.

ULOQs: GM-CSF, > 5,000 pg/mL; IFNγ, > 5,000 pg/mL; IL-1α, > 5,000 pg/mL;  IL-1β, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-5, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-13, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; IL-21, > 5,000 pg/mL; IL-23p19, > 5,000 pg/mL; KC, > 2,000 pg/mL;  TNFα, > 1,500 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

 For Research Use Only.  Not for use in diagnostic procedures.