PN: C281118

COMPONENTS:

1x Ab-conjugated beads (premixed). PN:  C281118A. One bottle containing 5 mL of the following AimPlex antibody-bead conjugates:

S4P2 - anti-M IL-2

S4P3 - anti-M IL-5

S4P4 - anti-M TNFalpha

S4P6 - anti-M IL-1beta

S4P7 - anti-M IL-12p70

S4P11 - anti-M IL-4

S4P12 - anti-M IL-17A

S5P2 - anti-M KC

S5P3 - anti-M GM-CSF

S5P4 - anti-M IFNgamma

S5P5 - anti-M IL-23p19

S5P6 - anti-M IL-22

S5P7 - anti-M IL-13

S5P8 - anti-TSLP

S5P9 - anti-M IL-10

S5P10 - anti-M IL-21

S5P11 - anti-M IL-1alpha

S5P12 - anti -M IL-6

2x Biotin-detection Ab (premixed). PN: C281118B. One vial containing 1.5 mL of the following biotinylated antibodies.

Biotin - anti-M IL-2

Biotin - anti-M IL-5

Biotin - anti-M TNFalpha

Biotin - anti-M IL-1beta

Biotin - anti-M IL-12p70

Biotin - anti-M IL-4

Biotin - anti-M IL-17A

Biotin - anti-M KC

Biotin - anti-M GM-CSF

Biotin - anti-M IFNgamma

Biotin - anti-M IL-23

Biotin - anti-M IL-22

Biotin - anti-M IL-13

Biotin - anti-TSLP

Biotin - anti-M IL-10

Biotin - anti-M IL-21

Biotin - anti-M IL-1alpha

Biotin - anti -M IL-6

2x Mouse/Rat dAb Diluent. PN: 07MRDX21. One vial containing 1.5 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Mouse TH1/Th2/Th17 18-Plex. PN: MG28018. One vial containing lyophilized recombinant GM-CSF, IFNγ, IL-1α, IL-1β, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22, IL-23, KC, TNFα. and TSLP. 

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels: 

      1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 3 mL of 1x SAPE.

      10x Reading Buffer. PN: 07RB1. One bottle containing 5 mL of 10x buffer.

      10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.

      PCR 8-tube Strip. PN: 07P8T1. 2 strips.

      Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

      Plate Seals. PN: 07PSR1. 6 sealers.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying the mouse Th1/Th2/Th17 18-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure. 

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): GM-CSF, < 5 pg/mL; IFNγ, < 0.5 pg/mL; IL-1α, < 2 pg/mL; IL-1β, < 2 pg/mL; IL-2, < 2 pg/mL; IL-4, < 0.5 pg/mL; IL-5, < 5 pg/mL; IL-6, < 3 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 1 pg/mL; IL-13, < 5 pg/mL; IL-17A, <1.5 pg/mL; IL-21, < 5 pg/mL; IL-22, < 5 pg/mL; IL-23p19, < 5 pg/mL; KC, < 1 pg/mL; TNFα, < 0.5 pg/mL; TSLP, < 2 pg/mL.

LLOQs: GM-CSF, < 10 pg/mL; IFNγ, < 2 pg/mL; IL-1α, < 10 pg/mL; IL-1β, < 5 pg/mL; IL-2, < 5 pg/mL; IL-4, < 2 pg/mL; IL-5, < 10 pg/mL; IL-6, < 10 pg/mL;  IL-10, < 5 pg/mL; IL-12p70, < 2 pg/mL; IL-13, < 10 pg/mL; IL-17A, < 5 pg/mL; IL-21, < 10 pg/mL; IL-22, < 10 pg/mL; IL-23p19, < 10 pg/mL; KC, < 2 pg/mL; TNFα, < 2 pg/mL; TSLP, < 5 pg/mL.

ULOQs: GM-CSF, > 5,000 pg/mL; IFNγ, > 5,000 pg/mL; IL-1α, > 5,000 pg/mL;  IL-1β, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-5, > 5,000 pg/mL; IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-13, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; IL-21, > 5,000 pg/mL; IL-22, > 5,000 pg/mL;  IL-23p19, > 5,000 pg/mL; KC, > 2,000 pg/mL;  TNFα, > 1,500 pg/mL; TSLP, < 5,000 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

 For Research Use Only.  Not for use in diagnostic procedures.