PN: C461107TK

COMPONENTS:

1x Ab-conjugated beads (premixed). PN: C461107TA. One bottle containing 1.5 mL of the following AimPlex antibody-bead conjugates:       

S4P3 - anti-NHP TNFα
S4P7 - anti-NHP IL-2
S4P9 - anti-NHP IFNγ
S5P9 - anti-NHP IL-17A
S5P4 - anti-NHP IL-4
S5P6 - anti-NHP IL-6
S5P10 - anti-NHP IL-10

2x Biotin-detection Ab (premixed). PN: C461107TB. One vial containing 0.45 mL of the following biotinylated antibodies.

Biotin - anti-NHP TNFα
Biotin - anti-NHP IL-2
Biotin - anti -NHP IFNγ
Biotin - anti-NHP IL-17A
Biotin - anti-NHP IL-4
Biotin - anti-NHP IL-6
Biotin - anti-NHP IL-10

2x Mouse/Rat dAb Diluent. PN: 07NDX21T. One vial containing 0.45 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- NHP Group 1 Panel A, 7-Plex. PN: NG10007. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα

Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying NHP Th1/Th2/Th17 7-Plex Panel. To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 32 Tests (PN: U100005T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): IFNγ, < 5 pg/mL; IL-2, < 5 pg/mL; IL-4 < 1 pg/mL, IL-6 <2 pg/mL; IL_10, < 2 pg/mL; IL-17A, < 2 pg/mL; TNFα, < 1 pg/mL

LLOQs: IFNγ, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4 < 5 pg/mL, IL-6 <5 pg/mL, IL-10, < 5 pg/mL; IL-17A, < 5 pg/mL; TNFα, < 2 pg/mL

ULOQs: IFNγ, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4 > 2,000 pg/mL; IL-6 > 5,000 pg/ml; IL-10, > 5,000 pg/mL; IL-17A, > 5,000 pg/mL; TNFα, > 5,000 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

 For Research Use Only.  Not for use in diagnostic procedures.