1x Ab-conjugated beads (premixed). PN: C371107A. One bottle containing 5 mL of the following AimPlex antibody-bead conjugates:
S4P4 - anti-rat IL-4
S4P6 - anti-rat IL-6
S4P9 - anti-rat IFNγ
S4P12 - anti-rat IL-2
S5P5 - anti-rat TNFα
S5P7 - anti-rat IL-17A
S5P10 - anti-rat IL-10
2x Biotin-detection Ab (premixed). PN: C371107B. One vial containing 1.5 mL of the following biotinylated antibodies.
Biotin - anti-rat IL-4
Biotin - anti-rat IL-6
Biotin - anti-rat IFNγ
Biotin - anti-rat IL-2
Biotin - anti-rat TNFα
Biotin - anti-rat IL-17A
Biotin - anti-rat IL-10
2x Mouse/Rat dAb Diluent. PN: 07MRDX21T. One vial containing 1.5 mL of biotin detection antibody diluent
Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. PN: RG10007. One vial containing lyophilized recombinant rat IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα.
Standard Info Sheet: Indicates reconstitute volume and standard concentrations.
Basic Kit for Premixed Panels:
1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 3 mL of 1x SAPE.
10x Reading Buffer. PN: 07RB1. One bottle containing 5 mL of 10x buffer.
10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.
PCR 8-tube Strip. PN: 07P8T1. 2 strips.
Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.
Plate Seals. PN: 07PSR1. 6 sealers.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying Rat Th1/Th2/Th17 7-Plex Panel. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit Premixed Panels (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD, pg/mL) LLOQ: pg/mL ULOQ pg/mL
Rat IFNg <2 <5 > 5,000
Rat IL-2 <2 <5 > 5,000
Rat IL-4 <2 <5 > 5,000
Rat IL-6 <2 <5 > 5,000
Rat IL-10 <2 <5 > 5,000
Rat IL-17A <2 <5 > 5,000
Rat TNFa <2 <5 > 5,000
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Rat Group 1: Negligible
Sample volume: 15 µL/test