PN: C371107K

COMPONENTS

1x Ab-conjugated beads (premixed). PN: C371107A. One bottle containing 5 mL of the following AimPlex antibody-bead conjugates:

S4P4 - anti-rat IL-4

S4P6 - anti-rat IL-6

S4P9 - anti-rat IFNγ

S4P12 - anti-rat IL-2

S5P5 - anti-rat TNFα

S5P7 - anti-rat IL-17A

S5P10 - anti-rat IL-10

2x Biotin-detection Ab (premixed). PN: C371107B. One vial containing 1.5 mL of the following biotinylated antibodies.

Biotin - anti-rat IL-4

Biotin - anti-rat IL-6

Biotin - anti-rat IFNγ

Biotin - anti-rat IL-2

Biotin - anti-rat TNFα

Biotin - anti-rat IL-17A

Biotin - anti-rat IL-10

2x Mouse/Rat dAb Diluent. PN: 07MRDX21T. One vial containing 1.5 mL of biotin detection antibody diluent

Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. PN: RG10007. One vial containing lyophilized recombinant rat IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα.  

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying Rat Th1/Th2/Th17 7-Plex Panel. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit Premixed Panels (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD, pg/mL)   LLOQ: pg/mL   ULOQ pg/mL

Rat IFNg         <2                     <5                   > 5,000

Rat IL-2          <2                     <5                   > 5,000

Rat IL-4          <2                     <5                   > 5,000

Rat IL-6          <2                     <5                   > 5,000

Rat IL-10         <2                     <5                   > 5,000

Rat IL-17A       <2                     <5                   > 5,000

Rat TNFa       <2                     <5                   > 5,000

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Rat Group 1: Negligible

Sample volume: 15 µL/test