96 Tests PN: B511127

Components:

45x Ab-conjugated beads (S5P11 - Canine IL-22 Ab-bead). PN: B511127A. One vial containing 100 µL of anti-canine IL-22 conjugated to AimPlex Bead S5P11.

25x Biotin-detection Ab (Canine IL-22 Biotin-dAb). PN: B511127B. One vial containing 100 µL of biotinylated anti Canine IL-22.

Lyophilized Standard -Canine IL-22. PN: B511127S. One vial containing lyophilized recombinant IL-22.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying Canine IL-22.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Interleukin 22 is a member of the IL10 superfamily, all of which express through cytokine receptor family class II. It is secreted by activated T and NK cells. The receptor that binds to IL-22 is expressed by non-hematopoietic cells, thus IL-22 acts as a signaling protein between lymphocytes and epithelial cells. It is also an important mediator in tissue repair and epithelial cell proliferation. Diseases associated with IL-22 expression are plaque psoriasis, Behcet’s disease, pneumonia, and asthma.

References:

  1. Honda K. IL-22 from T Cells: Better Late than Never. Immunity. 2012; 37(6): 952-954. Doi:10.1016/j.immuni.2012.11.006.

  2. Tufman A, Huber RM, Volk S, Aigner F, Edelmann M, Gamarra F, Kiefl R, Kahnert K, Tian F, Boulesteix A, Endres S, Kobold S. Interleukin-22 is elevated in lavage from patients with lung cancer and other pulmonary disease. BMC Cancer. 2016; 16(409): 1471-2407. Doi:10.1186./s12885-016-2471-2.

  3. Wook K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R. IL-22 Increases the Innate Immunity of Tissues. Immunity. 2004; 21 (2): 241-254. Doi:10.1016/j.immuni.2004.07.007.