PN: B514137

COMPONENTS

45x Ab-conjugated beads (S5P8 - Canine Insulin Ab-bead). PN: B514137A. One vial containing 100 µL of anti-canine Insulin conjugated to AimPlex Bead S5P8.

25x Biotin-detection Ab (Canine Insulin-dAb). PN: B514137B. One vial containing 100 µL of biotinylated anti-Canine Insulin.

Lyophilized Standard Mix-Canine Group Additional. PN: B514137S. One vial containing lyophilized insulin. 

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying Canine Insulin.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 µUnits/mL

Quantitation range:

LLOQ: < 30 µUnits/mL

ULOQ: > 2,000 µUnits/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Insulin (from Latin insula, island) is a peptide hormone produced by beta cells of the pancreatic islets, and it is considered to be the main anabolic hormone of the body. It regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of, especially, glucose from the blood into fat, liver and skeletal muscle cells. In these tissues the absorbed glucose is converted into either glycogen via glycogenesis or fats (triglycerides) via lipogenesis, or, in the case of the liver, into both. Glucose production and secretion by the liver is strongly inhibited by high concentrations of insulin in the blood. Dogs can have insulin-dependent, or Type 1, diabetes; research finds no Type 2 diabetes in dogs. Diabetes mainly affects middle-age and older dogs, but there are juvenile cases. The typical canine diabetes patient is middle-age, female, and overweight at diagnosis.

References:

1.     Alberti, KGMM; Aschner, P.; et al. (1999). "Definition, Diagnosis and Classification of Diabetes Mellitus". World Health Organization. Retrieved 17 March 2010.

2.     Catchpole, B.; Ristic, J. M.; Fleeman, L. M.; Davison, L. J. (8 September 2005). "Canine diabetes mellitus: can old dogs teach us new tricks?". Diabetologia. 48 (10): 1948–1956. doi:10.1007/s00125-005-1921-1.

3.     Foster, Race. "Juvenile Onset Diabetes Mellitus (Sugar Diabetes) in Dogs & Puppies". Drs. Foster & Smith-Pet Education.

4.     Bruyette, David (2001). "Diabetes Mellitus: Treatment Options". World Small Animal Veterinary Association (WSAVA).