45x Ab-conjugated beads (S5P10 - Canine SCF Ab-bead). PN: B511110A. One vial containing 100 µL of anticanine SCF conjugated to AimPlex Bead S5P10.
25x Biotin-detection Ab (Canine SCF Biotin-dAb). PN: B511110B. One vial containing 100 µL of biotinylated anticanine SCF.
Lyophilized Standard Mix-Canine Group 1 Panel A, 10-Plex. PN: KG10010. One vial containing lyophilized recombinant IFNγ, IL-2, IL-6, IL-8, IL-10, IL-12p40, IL-17A, MCP-1, SCF and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying Canine SCF/MGF/KITLG. Can be multiplexed with other analytes in Canine Group 1. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
LLOQ: < 20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Canine Group 1: Negligible
Sample volume: 15 µL/test
Stem cell factor (SCF), also known as mast cell growth factor (MGF), KIT-ligand and Steel Factor, is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF plays an important role in the hematopoiesis during embryonic development. Sites where hematopoiesis takes place, such as the fetal liver and bone marrow, all express SCF. Mice that do not express SCF die in utero from severe anemia. Mice that do not express the receptor for SCF (c-KIT) also die from anemia. SCF may serve as guidance cues that direct hematopoietic stem cells (HSCs) to their microenvironment in which a stem cell resides and it plays an important role in HSC maintenance. Diseases associated with KITLG include hyperpigmentation, familial progressive, 2 and testicular non seminomatous germ cell tumor.
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