• PN: A113248

COMPONENTS

45x Ab-conjugated beads (S5P5 - human Adiponectin Ab-bead). PN: A113248A. One vial containing   100 µL of anti-human Adiponectin conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (human Adiponectin Biotin-dAb). PN: A113248B. One vial containing   100 µL of biotinylated anti-human Adiponectin.

Lyophilized Standard Mix - Human Adiponectin. PN: A113248S. One vial containing lyophilized Adiponectin.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human Adiponectin. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Important Note: Some specimens may contain high levels of adiponetin.  For normal serum and plasma samples, pre-diluting samples 500 folds with the Sample Dilution Buffer (PN: P830100) is recommended.  Dilution factors should be optimized according to specific experimental conditions and sample types.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 20,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Adiponectin, also known as Acrp30, is a hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation.  Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. Levels of the hormone are inversely correlated with body fat percentage in adults; however, a meta analysis was not able to confirm this association in healthy adults. The association in infants and young children is less clear. Similarly, circulating adiponectin concentrations increase during caloric restriction in animals and humans, such as in patients with anorexia nervosa. This observation is surprising, given that adiponectin is produced by adipose tissue; however, a recent study suggests that adipose tissue within bone marrow, which increases during caloric restriction, contributes to elevated circulating adiponectin in this context. Diseases associated with Acrp30 include adiponectin deficiency and chronic pulmonary heart disease.

References:

1.       Díez JJ, Iglesias P (2003). "The role of the novel adipocyte-derived hormone adiponectin in human disease". Eur. J. Endocrinol. 148 (3): 293–300. PMID 12611609.

2.       Chen J, Tan B, Karteris E, Zervou S, Digby J, Hillhouse EW, Vatish M, Randeva HS (2006). "Secretion of adiponectin by human placenta: differential modulation of adiponectin and its receptors by cytokines". Diabetologia 49 (6): 1292–302. PMID 16570162.

3.       Ukkola O, Santaniemi M (2002). "Adiponectin: a link between excess adiposity and associated comorbidities?" J. Mol. Med. 80 (11): 696–702. PMID 12436346.

4.       Kuo SM, Halpern MM (2011). "Lack of association between body mass index and plasma adiponectin levels in healthy adults". Int J Obes (Lond) 35 (12): 1487–94. PMID 21364526.

5.       Cawthorn WP, Scheller EL, Learman BS, Parlee SD, et.al. (2014). "Bone marrow adipose tissue is an endocrine organ that contributes to increased circulating adiponectin during caloric restriction". Cell Metab. 20 (2): 368–375. PMID 24998914.