- PN: B111465
45x Ab-conjugated beads (S4P6 - human AFP Ab-bead). PN: B111465A. One vial containing 100 µL of anti-human AFP conjugated to AimPlex Bead S4P6.
25x Biotin-detection Ab (human AFP Biotin-dAb). PN: B111465B. One vial containing 100 µL of biotinylated anti-human AFP.
Lyophilized Standard Mix - Human AFP. PN: B111465S. One vial containing lyophilized AFP.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human Human AFP/Alpha-Fetoprotein. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 20,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Sample volume: 15 µL/test
Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. AFP is measured in pregnant women through the analysis of maternal blood or amniotic fluid, as a screening test for a subset of developmental abnormalities. Some of the diseases in which AFP will be elevated are omphalocele, hepatocellular carcinoma,neural tube defects, nonseminomatous germ cell tumors, yolk sac tumor, ataxia telangiectasia and can also be used as a biomarker to detect a subset of tumors in non-pregnant women, men, and children with levels above 500 nanograms/milliliter of AFP in adults indicative of hepatocellular carcinoma, germ cell tumors, and metastatic cancers of the liver.
1. Szabó M, Veress L, Münnich A, Papp Z (September 1990). "[Alpha fetoprotein concentration in the amniotic fluid in normal pregnancy and in pregnancy complicated by fetal anomaly]". Orv Hetil (in Hungarian) 131 (39): 2139–42. PMID 1699194.
2. Rosen T, D'Alton ME (December 2005). "Down syndrome screening in the first and second trimesters: what do the data show?". Semin. Perinatol. 29 (6): 367–75. doi:10.1053/j.semperi.2006.01.001. PMID 16533649.
3. Bredaki FE, Poon LC, Birdir C, Escalante D, Nicolaides KH (2012). "First-trimester screening for neural tube defects using alpha-fetoprotein". Fetal. Diagn. Ther. 31 (2): 109–14. doi:10.1159/000335677. PMID 22377693.
4. Taylor AM, Byrd PJ (October 2005). "Molecular pathology of ataxia telangiectasia". J. Clin. Pathol. 58 (10): 1009–15. doi:10.1136/jcp.2005.026062. PMC 1770730. PMID 16189143.