• PN: A11735

COMPONENTS

45x Ab-conjugated beads (S5P5- Human Amphiregulin Ab-bead). PN: A117355A. One vial containing 100 µL of anti-human Amphiregulin conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (human Amphiregulin Biotin-dAb). PN: A117355B. One vial containing 100 µL of biotinylated anti-human Amphiregulin.

Lyophilized Standard Mix - Human Amphiregulin. PN: A117355S. One vial containing lyophilized AFP.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human Amphiregulin/AREG. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Amphiregulin, also known as AREG, is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. Estradiol and progesterone mostly induce amphiregulin expression to mediate ductal development of the mammary glands. Amphiregulin has been found to be essential for mammary ductal development, as evidenced by absence of ductal growth in amphiregulin knockout mice. This is similar to the phenotypes of EGFR and ERα knockout mice, which also show absence of ductal growth. Diseases associated with AREG include Psoriasis and Colorectal Cancer.

References:

1.     Shoyab M, Plowman GD, McDonald VL, Bradley JG, Todaro GJ (February 1989). “Structure and function of human amphiregulin: a member of the epidermal growth factor family”. Science. 243 (4894 Pt 1): 1074–1076. PMID 2466334. doi:10.1126/science.2466334.

2.     Plowman GD, Green JM, McDonald VL, Neubauer MG, Disteche CM, Todaro GJ, Shoyab M (May 1990). “The amphiregulin gene encodes a novel epidermal growth factor-related protein with tumor-inhibitory activity”. Molecular and Cellular Biology. 10 (5): 1969–1981. PMC 360543 Freely accessible. PMID 2325643.

3.     Aupperlee MD, Leipprandt JR, Bennett JM, Schwartz RC, Haslam SZ (2013). “Amphiregulin mediates progesterone-induced mammary ductal development during puberty”. Breast Cancer Research. 15 (3): R44. PMC 3738150 Freely accessible. PMID 23705924. doi:10.1186/bcr3431.

4.     LaMarca HL, Rosen JM (2007). “Estrogen regulation of mammary gland development and breast cancer: amphiregulin takes center stage”. Breast Cancer Research. 9 (4): 304. PMC 2206713 Freely accessible. PMID 17659070. doi:10.1186/bcr1740.