• PN: B111362

COMPONENTS

45x Ab-conjugated beads (S4P9 - human BDNF Ab-bead). PN: B111362A. One vial containing 100 µL of anti-human BDNF conjugated to AimPlex Bead S4P9.

25x Biotin-detection Ab(human BDNF Biotin-dAb). PN: B111362B. One vial containing 100 µL of biotinylated anti-human BDNF.

Lyophilized Standard Mix-Human Group 4 Panel A. PN: HG4009A. One vial containing lyophilized recombinant human BDNF, beta-NGF, EGF, FGF basic, OPG, OPN, PDGF-AB and VEGF-C.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human BDNF.  Can be multiplexed with other analytes in Human Group 4.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 4: Negligible

Sample volume: 15 µL/test

Description:

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. Neurotrophic factors are found in the brain and the periphery. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. It is also expressed in the retina, motor neurons, the kidneys, saliva, and the prostate. BDNF itself is important for long-term memory. Neurotrophins are proteins that help to stimulate and control neurogenesis, BDNF being one of the most active.  Diseases associated with BDNF include bulimia nervosa, age of onset of weight loss in and anorexia nervosa

References:

1.      Robinson RC, Radziejewski C, Stuart DI, Jones EY (April 1995). "Structure of the brain-derived neurotrophic factor/neurotrophin 3 heterodimer". Biochemistry 34 (13): 4139–46. doi:10.1021/bi00013a001. PMID 7703225.

2.      Binder DK, Scharfman HE (Sep 2004). "Brain-derived neurotrophic factor". Growth Factors 22 (3): 123–31. doi:10.1080/08977190410001723308. PMC 2504526. PMID 15518235.

3.      Jones KR, Reichardt LF (Oct 1990). "Molecular cloning of a human gene that is a member of the nerve growth factor family". Proceedings of the National Academy of Sciences of the United States of America 87 (20): 8060–4. Bibcode:1990PNAS...87.8060J. doi:10.1073/pnas.87.20.8060. PMC 54892. PMID 2236018.

4.      Maisonpierre PC, Le Beau MM, Espinosa R, Ip NY, Belluscio L, de la Monte SM, Squinto S, Furth ME, Yancopoulos GD (Jul 1991). "Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations". Genomics 10 (3): 558–68. doi:10.1016/0888-7543(91)90436-I. PMID 1889806.