• PN: B115584

COMPONENTS

45x Ab-conjugated beads (S4P4 - Human BMP-4 Ab-bead). PN: B115584A. One vial containing 100 µL of anti-Human BMP-4 conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Human BMP-4 Biotin-dAb). PN: B115584B. One vial containing 100 µL of biotinylated anti-Human BMP-4.

Lyophilized Standard Mix-Human Group 8, Panel A, 7-Plex. PN: HG81007. One vial containing lyophilized recombinant BMP-2, BMP-4, IGFBP-3, IGFBP-5, IGFBP-6, TIMP-1, and TIMP-2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human BMP-4. Can be multiplexed with other analytes in Human Group 8.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 8: Negligible

Sample volume: 15 µL/test

Description:

BMP-4 (BMP4) is a polypeptide and a member of the bone morphogenetic protein family, which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. BMP4 is found in early embryonic development in the ventral marginal zone and in the eye, heart blood and otic vesicle. BMP-4, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. This particular family member plays an important role in the onset of endochondral bone formation in humans. It has been shown to be involved in muscle development, bone mineralization, and ureteric bud development. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. Increase in expression of BMP4 has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.  Diseases associated with BMP4 include microphthalmia, syndromic 6 and orofacial cleft 11.

References:

1.      van den Wijngaard A, Weghuis DO, Boersma CJ, van Zoelen EJ, Geurts van Kessel A, Olijve W (Nov 1995). "Fine mapping of the human bone morphogenetic protein-4 gene (BMP4) to chromosome 14q22-q23 by in situ hybridization". Genomics 27 (3): 559–60. doi:10.1006/geno.1995.1096. PMID 7558046.

2.      Oida S, Iimura T, Maruoka Y, Takeda K, Sasaki S (Nov 1995). "Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library". DNA Seq 5 (5): 273–5. doi:10.3109/10425179509030980. PMID 7579580.

3.      Kan L, Hu M, Gomes WA, Kessler JA (October 2004). "Transgenic Mice Overexpressing BMP4 Develop a Fibrodysplasia Ossificans Progressiva (FOP)-Like Phenotype". Am. J. Pathol. 165 (4): 1107–15. doi:10.1016/S0002-9440(10)63372-X. PMC 1618644. PMID 15466378.

4.      Dixon MJ, Marazita ML, Beaty TH, Murray JC (March 2011). "Cleft lip and palate: understanding genetic and environmental influences". Nat. Rev. Genet. 12 (3): 167–78. doi:10.1038/nrg2933. PMC 3086810. PMID 21331089.