• PN: B115593

COMPONENTS

45x Ab-conjugated beads (S5P5 - Human CA125 ab-bead). PN: B115593A. One vial containing 100 µL of anti-human CA125 conjugated to AimPlex Bead S5P5

25x Biotin-detection Ab (Human CA125 Biotin-dAb). PN: B115593B. One vial containing 100 µL of biotinylated anti-human CA125.

Lyophilized Standard Mix - Human CA125. PN: B115593S. One vial containing lyophilized CA125.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human CA125. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 Unit/mL

Quantitation range:

LLOQ: < 10 Unit/mL

ULOQ: > 5,000 Unit/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

CA125 (cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) also known as mucin 16 or MUC16 is a member of the mucin family glycoproteins. CA125 has found application as a tumor marker or biomarker that may be elevated in the blood of some patients with specific types of cancers, or other benign conditions. CA125 is the most frequently used biomarker for ovarian cancer detection. Around 90% of women with advanced ovarian cancer have elevated levels of CA-125 in their blood serum, making CA-125 a useful tool for detecting ovarian cancer after the onset of symptoms. CA-125 has limited specificity for ovarian cancer because elevated CA-125 levels can be found in individuals without ovarian cancer. For example, while CA-125 is best known as a marker for ovarian cancer, it may also be elevated in other cancers, including endometrial cancer, fallopian tube cancer, lung cancer, breast cancer and gastrointestinal cancer.

References:

1. Yin BW, Lloyd KO (Jul 2001). "Molecular cloning of the CA125 ovarian cancer antigen: identification as a new mucin, MUC16". The Journal of Biological Chemistry 276 (29): 27371–5. doi:10.1074/jbc.M103554200. PMID 11369781.

2. Yin BW, Dnistrian A, Lloyd KO (Apr 2002). "Ovarian cancer antigen CA125 is encoded by the MUC16 mucin gene". International Journal of Cancer. Journal International Du Cancer 98 (5): 737–40. doi:10.1002/ijc.10250. PMID 11920644.

3. Duraisamy S, Ramasamy S, Kharbanda S, Kufe D (May 2006). "Distinct evolution of the human carcinoma-associated transmembrane mucins, MUC1, MUC4 AND MUC16". Gene 373: 28–34. doi:10.1016/j.gene.2005.12.021. PMID 16500040.

4. Bast RC, Xu FJ, Yu YH, Barnhill S, Zhang Z, Mills GB (1998). "CA 125: the past and the future". The International Journal of Biological Markers 13 (4): 179–87. PMID 10228898.