• PN: B113355

COMPONENTS

45x Ab-conjugated beads (S5P5 - Human sCD163 Ab-bead). PN: B113355A. One vial containing 100 µL of anti-human sCD163 conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (Human sCD163 Biotin-dAb). PN: B113355B. One vial containing 100 µL of biotinylated anti- Human sCD163.

Lyophilized Standard Mix - Human sCD163. PN: HCD163AG. One vial containing lyophilized sCD163.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human CD163/M130/sCD163. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 30 pg/mL

Quantitation range:

LLOQ: < 60 pg/mL

ULOQ: > 10,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

CD163 (Cluster of Differentiation 163) is the high affinity scavenger receptor for the hemoglobin-haptoglobin complex, and in the absence of haptoglobin with lower affinity, for hemoglobin alone. It has also been shown to mark cells of monocyte/macrophage lineage.  A soluble form of the receptor exists in plasma, commonly denoted sCD163. It is generated by ectodomain shedding of the membrane bound receptor. sCD163 is upregulated in a large range of inflammatory diseases including liver cirrhosis, type 2 diabetes, macrophage activation syndrome, Gaucher's disease, sepsis, HIV infection, rheumatoid arthritis and Hodgkin Lymphoma.

References:

1.       CD163 is the macrophage scavenger receptor for native and chemically modified hemoglobins in the absence of haptoglobin. Schaer DJ, Schaer CA, Buehler PW, Boykins RA, Schoedon G, Alayash AI, Schaffner A. Blood. 2006 Jan 1;107(1):373-80. PMID 16189277

2.       Lau SK, Chu PG, Weiss LM (2004). "CD163: a specific marker of macrophages in paraffin-embedded tissues.". Am. J. Clin. Path. 122 (5): 794–801. doi:10.1309/QHD6YFN81KQXUUH6. PMID 15491976.

3.       Onofre G, Kolácková M, Jankovicová K, Krejsek J (2009). "Scavenger receptor CD163 and its biological functions". Acta Medica (Hradec Kralove) 52 (2): 57–61. PMID 19777868.

4.       Jones K, Vari F, Keane C, Crooks P, Nourse JP, Seymour LA, Gottlieb D, Ritchie D, Gill D, Gandhi MK (February 2013). "Serum CD163 and TARC as disease response biomarkers in classical Hodgkin lymphoma". Clin. Cancer Res. 19 (3): 731–42. doi:10.1158/1078-0432.CCR-12-2693. PMID 23224400.

5.       Møller HJ (February 2012). "Soluble CD163". Scand. J. Clin. Lab. Invest. 72 (1): 1–13. doi:10.3109/00365513.2011.626868. PMID 22060747.