• PN: B113330

COMPONENTS

45x Ab-conjugated beads (S4P4 - human sCD40 Ab-bead). PN: B113330A. One vial containing 100 µL of anti-human sCD40 conjugated to AimPlex Bead S4P4.

25x Biotin-detection (human sCD40 Biotin-dAb). PN: B113330B. One vial containing 100 µL of biotinylated anti-human sCD40.

Lyophilized Standard Mix-Human Group 9 Panel A, 8-Plex. PN: HG9008A. One vial containing lyophilized recombinant human sFas, sICAM-3,  sL-selectin, sP-selectin, sIL-1RI, sVEGFR1, sCD40, and sNCAM-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human TNFRSF5/sCD40. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 9: Negligible

Sample volume: 15 µL/test

Description:

Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5) is a member of the TNF-receptor superfamily is also known as CD 40 antigen. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Diseases associated with CD40 include immunodeficiency with hyper-IgM, type 3 and CD40 deficiency.

References:

1.      Chatzigeorgiou A, Lyberi M, Chatzilymperis G, Nezos A, Kamper E (2009). "CD40/CD40L signaling and its implication in health and disease". BioFactors (Oxford, England) 35 (6): 474–83. doi:10.1002/biof.62. PMID 19904719.

2.      McWhirter SM, Pullen SS, Holton JM, Crute JJ, Kehry MR, Alber T (Jul 1999). "Crystallographic analysis of CD40 recognition and signaling by human TRAF2". Proceedings of the National Academy of Sciences of the United States of America 96 (15): 8408–13. doi:10.1073/pnas.96.15.8408. PMC 17529. PMID 10411888.

3.      Tsukamoto N, Kobayashi N, Azuma S, Yamamoto T, Inoue J (Feb 1999). "Two differently regulated nuclear factor kappaB activation pathways triggered by the cytoplasmic tail of CD40". Proceedings of the National Academy of Sciences of the United States of America 96 (4): 1234–9. doi:10.1073/pnas.96.4.1234. PMC 15446. PMID 9990007.

4.      Malinin NL, Boldin MP, Kovalenko AV, Wallach D (Feb 1997). "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1". Nature 385 (6616): 540–4. doi:10.1038/385540a0. PMID 9020361.