96 Tests PN: A113257

Components:

45x Ab-conjugated beads (S5P2 - human sCD95L Ab-bead). PN: A113257A. One vial containing 100 µL of anti-human sCD95L conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (human sCD95L Biotin-dAb). PN: A113257B. One vial containing 100 µL of biotinylated anti-human sCD95L.

Lyophilized Standard Mix-Human Group 5 Panel B, 10-Plex. PN: HG50010. One vial containing lyophilized recombinant human sRANKL, sEndoglin, sVCAM-1, sE-selectin, sCD95L, sICAM-1, sCD25, sCD14, sTNFRI, and sCD40L.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human TNFSF6/sCD178/sCD95L/FasL.  Can be multiplexed with other analytes in Human Group 5.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 2 pg/mL

  • ULOQ: > 2,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 5: Negligible

  • Sample volume: 15 µL/test

Description:

Tumor Necrosis Factor Receptor SuperFamily, Member 5 (TNFSF5), also called CD154 or CD40 ligand (CD40L), is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on T follicular helper cells (TFH cells). On TFH cells, CD154 promotes B cell maturation and function by engaging CD40 on the B cell surface and therefore facilitating cell-cell communication. A defect in this gene results in an inability to undergo immunoglobulin class switching and is associated with hyper IgM syndrome. Absence of CD154 also stops the formation of germinal centers and therefore prohibiting antibody affinity maturation, an important process in the adaptive immune system.

References:

  1. Sheikh MS, Fornace AJ (2000). "Death and decoy receptors and p53-mediated apoptosis". Leukemia 14 (8): 1509–1513. doi:10.1038/sj.leu.2401865. PMID 10942251.

  2. Andersen MH, Schrama D, Thor Straten P, Becker JC (2006). "Cytotoxic T cells". J. Invest. Dermatol. 126 (1): 32–41. doi:10.1038/sj.jid.5700001. PMID 16417215.

  3. Igney FH, Krammer PH (2005). "Tumor counterattack: fact or fiction?". Cancer Immunol. Immunother. 54 (11): 1127–1136. doi:10.1007/s00262-005-0680-7. PMID 15889255.

  4. Gajate C, Mollinedo F (March 2005). "Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy". J. Biol. Chem. 280 (12): 11641–7. doi:10.1074/jbc.M411781200. PMID 15659383.