• PN: B115603

COMPONENTS

45x Ab-conjugated beads (S5P6 - Human CEA Ab-bead). PN: B115603A. One vial containing 100 µL of anti-human CEA conjugated to AimPlex Bead S5P6.

25x Biotin-detection Ab (Human CEA Biotin-dAb). PN: B115603B. One vial containing 100 µL of biotinylated anti-human CEA.

Lyophilized Standard Mix - Human CEA. PN: B115603S. One vial containing lyophilized CEA.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human CEA. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 1,000 pg/mL

Quantitation range:

LLOQ: < 2,000 pg/mL

ULOQ: > 500,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Carcinoembryonic antigen (CEA) describes a set of highly related glycoproteins involved in cell adhesion. Immunologically they are characterized as an antigen of the CD66 cluster of differentiation (CD66e). CEA is normally produced in gastrointestinal tissue during fetal development, but the production stops before birth. CEA is usually present only at very low levels in the blood of healthy adults, however, the serum levels are raised in some types of cancer, which means that it can be used as a tumor marker in clinical tests. Serum levels can also be elevated in heavy smokers. CEA are glycosyl phosphatidyl inositol (GPI) cell surface anchored glycoproteins whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and Eselectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. CEA measurement is mainly used as a tumor marker to monitor colorectal carcinoma treatment, to identify recurrences after surgical resection, for staging or to localize cancer spread through measurement of biological fluids. CEA levels may also be raised in gastric carcinoma, pancreatic carcinoma, lung carcinoma, breast carcinoma, and medullary thyroid carcinoma, as well as some non-neoplastic conditions like ulcerative colitis, pancreatitis, cirrhosis, COPD, Crohn's disease, and hypothyroidism.

References:

1. Boehm, M. K.; Perkins, S. J. (2000). "Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23". FEBS Letters 475 (1): 11–16. doi:10.1016/S0014-5793(00)01612-4. PMID 10854848.

2. Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K (Jun 2008). "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow". J Biol Chem 283 (23): 15647–55. doi:10.1074/jbc.M800543200. PMC 2414264. PMID 18375392.

3. Konstantopoulos K, Thomas SN (2009). "Cancer cells in transit: the vascular interactions of tumor cells". Annu Rev Biomed Eng 11: 177–202. doi:10.1146/annurev-bioeng-061008-124949. PMID 19413512.

4. Thomas SN, Tong Z, Stebe KJ, Konstantopoulos K (2009). "Identification, characterization and utilization of tumor cell selectin ligands in the design of colon cancer diagnostics". Biorheology 46 (3): 207–25. doi:10.3233/BIR-2009-0534. PMID 19581728.