• PN: B112471

COMPONENTS

45x Ab-conjugated beads (S4P12 - Human CXCL16 Ab-bead). PN: B112471A. One vial containing 100 µL of anti-Human CXCL16 conjugated to AimPlex Bead S4P12.

25x Biotin-detection Ab (Human CXCL16 Biotin-dAb). PN: B112471B. One vial containingv100 µL of biotinylated anti-Human CXCL16.

Lyophilized Standard Mix-Human Group 6, Panel A, 7-Plex. PN: HG61007. One vial containing lyophilized recombinant 6Ckine, BRAK, BCA-1, CTACK, CCL28, ENA-78, and CXCL16.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human CXCL16. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 6: Negligible

Sample volume: 15 µL/test

Description:

Chemokine (C-X-C motif) ligand 16 (CXCL16) or small-inducible cytokine B16 (SCYB16) is a small cytokine belonging to the CXC chemokine family. CXCL16 is expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The administration of folinic acid, which forces the methylation of CXCL 16, induces high levels of methylation of the CXCL 16 gene promoter in colon, ileum and lung and causes iNKT cells accumulation in these tissues. Colonization of neonatal GF mice, but not in adult mice, with a normal microbiota decreases hypermethylation levels of CXCL 16. Diseases associated with CXCL16 include Xantho-granulomatous cholecystitis and Cholecystitis.

References:

1.      Matloubian M, David A, Engel S, Ryan J, Cyster J (2000). "A transmembrane CXC chemokine is a ligand for HIV-coreceptor Bonzo". Nat Immunol 1 (4): 298–304. doi:10.1038/79738. PMID 11017100.

2.      Abel S, Hundhausen C, Mentlein R, Schulte A, Berkhout T, Broadway N, Hartmann D, Sedlacek R, Dietrich S, Muetze B, Schuster B, Kallen K, Saftig P, Rose-John S, Ludwig A (2004). "The transmembrane CXC-chemokine ligand 16 is induced by IFN-gamma and TNF-alpha and shed by the activity of the disintegrin-like metalloproteinase ADAM10". J Immunol 172 (10): 6362–72. doi:10.4049/jimmunol.172.10.6362. PMID 15128827.

3.      Wilbanks A, Zondlo S, Murphy K, Mak S, Soler D, Langdon P, Andrew D, Wu L, Briskin M (2001). "Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines". J Immunol 166 (8): 5145–54. doi:10.4049/jimmunol.166.8.5145. PMID 11290797.

4.      Olszak T, An D, Zeissig S, Vera MP, Richter J, Franke A, Glickman JN, Siebert R, Baron RM, Kasper DL, Blumberg RS (2012). "Microbial exposure during early life has persistent effects on natural killer T cell function". Science 336 (6080): 489–493. doi:10.1126/science.1219328. PMC 3437652. PMID 22442383.

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