• PN: B115605

COMPONENTS

45x Ab-conjugated beads (S5P9- Human Cystatin C Ab-bead). PN: B115605A. One vial containing 100 µL of anti-Human Cystatin C conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Human Cystatin C Biotin-dAb). PN: B115605B. One vial containing 100 µL of biotinylated anti-Human Cystatin C.

Lyophilized Standard Mix-Human Group 8, Panel B, 11-Plex. PN: HG82011. One vial containing lyophilized recombinant Cystatin C, FGF-1, FGF-19, Flt-3L, GDF-15, Granzyme B, NGAL, PAPP-A, SLPI, RAGE, and ST2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Cystatin C/CST3. Can be multiplexed with other analytes in Human Group 8.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 40 pg/mL

ULOQ: > 20,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 4: Negligible

Sample volume: 15 µL/test

Description:

Cystatin C or cystatin 3 (CST3), formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. In humans, all cells with a nucleus produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases and probably one of the most important extracellular inhibitors of cysteine proteases. Diseases associated with CST3 include macular degeneration, age-related, 11 and cerebral amyloid angiopathy.

References:

1.      Kolodziejczyk, R.; Michalska, K.; Hernandez-Santoyo, A.; Wahlbom, M.; Grubb, A.; Jaskolski, M. (2010). "Crystal structure of human cystatin C stabilized against amyloid formation". FEBS Journal 277 (7): 1726–1737. doi:10.1111/j.1742-4658.2010.07596.x. PMID 20175878.

2.      Zahran A, El-Husseini A, Shoker A (2007). "Can cystatin C replace creatinine to estimate glomerular filtration rate? A literature review". Am. J. Nephrol. 27 (2): 197–205. doi:10.1159/000100907. PMID 17361076.

3.      Roos JF, Doust J, Tett SE, Kirkpatrick CM (March 2007). "Diagnostic accuracy of cystatin C compared to serum creatinine for the estimation of renal dysfunction in adults and children--a meta-analysis". Clin. Biochem. 40 (5–6): 383–391. doi:10.1016/j.clinbiochem.2006.10.026. PMID 17316593.