PN: B117737

COMPONENTS

45x Ab-conjugated beads (S5P2- Human Elafin Ab-bead). PN: B117737A. One vial containing 100 µL of anti-human Elafin conjugated to AimPlex Bead S5P2.

25x Biotin-detection Ab (human Elafin Biotin-dAb). PN: B117737B. One vial containing 100 µL of biotinylated anti-human Elafin.

Lyophilized Standard -Human Elafin. PN: B117737S. One vial containing lyophilized Elafin. 

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Elafin.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 50 pg/mL

ULOQ: > 10,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Elafin, also known as peptidase inhibitor 3 (PI3) or skin-derived antileukoprotease (SKALP), has 38% identity with the second domain of secretory leukocyte proteinase inhibitor. Elafin has been found to have utility in serving as a biomarker for graft versus host disease of the skin and plays some role in gut inflammation, typical of celiac disease (gluten intolerance).

References:

1.      Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994). "Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12→q13". Cytogenet Cell Genet. 66 (2): 129–31. doi:10.1159/000133683. PMID 8287685.

2.      Clauss A, Lilja H, Lundwall A (Nov 2002). "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology

to whey acidic protein". Biochem J. 368 (Pt 1): 233–42. doi:10.1042/BJ20020869. PMC 1222987 Freely accessible. PMID 12206714.

3.      Paczesny S, et.al. (February 2009). "Elafin is a Biomarker of Graft Versus Host Disease of the Skin". Biology of Blood and Marrow Transplantation. 15 (2 Suppl 1): 13–14. doi:10.1016/j.bbmt.2008.12.039.

4.      Heather J. Galipeau, et.al. Novel Role of the Serine Protease Inhibitor Elafin in Gluten-Related Disorders. American Journal of Gastroenterology, April 2014 DOI: 10.1038/ajg.2014.48