• PN: B115608

COMPONENTS

45x Ab-conjugated beads (S4P4- Human Endostatin Ab-bead). PN: B115608A. One vial containing 100 µL of anti-human Endostatin conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Human Endostatin Biotin-dAb). PN: B115608B. One vial containing    100 µL of biotinylated anti- Human Endostatin.

Lyophilized Standard Mix - Human Endostatin. PN: B115608S. One vial containing lyophilized Endostatin.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human Endostatin. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Important Note: Some specimens may contain high level of endostatin. Pre-diluting normal serum and plasma samples 30 folds with the Sample Dilution Buffer (PN: P830100) is recommended for the endostatin assay. Dilution factors should be optimized according to specific experimental conditions and sample types.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 30 pg/mL

Quantitation range:

LLOQ: < 70 pg/mL

ULOQ: > 30,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Endostatin is a broad-spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). Collagen is a component of epithelial and endothelial basement membranes. Endostatin, as a fragment of collagen 18, demonstrates a role of the ECM in suppression of neoangiogenesis. Pro-angiogenic and anti-angiogenic factors can also be created by proteolysis during coagulation cascades. Endogenous inhibitors of angiogenesis are present in both normal tissue and cancerous tissue. Overall, endostatin down regulates many signaling cascades like ephrin, TNF-α, and NFκB signaling as well as coagulation and adhesion cascades. Diseases associated with COL18A1 include Knobloch Syndrome, Type 1 and Knobloch Syndrome.

References:

1.       Folkman, J. (2006). "Antiangiogenesis in cancer therapy--endostatin and its mechanisms of action". Exp. Cell. Res. 312 (5, part 2): 594–607. doi:10.1016/j.yexcr.2005.11.015. PMID 16376330.

2.       OReilly, M.S.; Boehm, T.; Shing, Y.; Fukai, N.; Vasios, G.; Lane, W.W.; Flynn, E.; Birkhead, J.R.; Olsen, B.R.; Folkman, J. (1997). "Endostatin: an endogenous inhibitor of angiogenesis and tumor growth". Cell. 88 (2): 277–85. doi:10.1016/S0092-8674(00)81848-6. PMID 9008168.

3.       Standker, L; Schrader, M.; Kanse, S.M.; Jurgens, M.; Forssmann, W.G.; Prissner, K.T. (1997). "Isolation and characterization of the circulating form of human endostatin". FEBS Lett. 420 (2–3): 129–33. doi:10.1016/S0014-5793(97)01503-2. PMID 9459295.

4.       Felbor, U; et al. (2000). "Secreted cathepsin L generates endostatin from collagen XVIII". EMBO J. 19 (6): 1187–94.