• PN: A113260

COMPONENTS

45x Ab-conjugated beads (S5P3 - human sFas Ab-bead). PN: A113260A. One vial containing 100 µL of anti-human sFas conjugated to AimPlex Bead S5P3.

25x Biotin-detection (human sFas Biotin-dAb). PN: A113260B. One vial containing 100 µL of biotinylated anti-human sFas.

Lyophilized Standard Mix-Human Group 9 Panel A, 8-Plex. PN: HG9008A. One vial containing lyophilized recombinant human sFas, sICAM-3, sL-selectin, sP-selectin, sIL-1RI, sVEGFR1, sCD40, and sNCAM-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human TNFRSF6/sCD95/sFasR. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 50 pg/mL

ULOQ: > 20,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 9: Negligible

Sample volume: 15 µL/test

Description:

Tumor Necrosis factor Receptor SuperFamily Member 6 (TNFRSF6), also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT) or CD95, is a death receptor on the surface of cells that leads to apoptosis. It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. FasR forms the death-inducing signaling complex upon ligand binding. Membrane-anchored FasR ligand trimer on the surface of an adjacent cell causes oligomerization of Fas. Some reports have suggested that the extrinsic Fas pathway is sufficient to induce complete apoptosis in certain cell types through DISC assembly and subsequent caspase-8 activation. These cells are dubbed Type 1 cells and are characterized by the inability of anti-apoptotic members of the Bcl-2 family (namely Bcl-2 and Bcl-xL) to protect from Fas-mediated apoptosis. Diseases associated with FAS include autoimmune lymphoproliferative syndrome and fas-related autoimmune lymphoproliferative syndrome.

References:

1.      Lichter P, Walczak H, Weitz S, Behrmann I, Krammer PH (September 1992). "The human APO-1 (APT) antigen maps to 10q23, a region that is syntenic with mouse chromosome 19". Genomics 14 (1): 179–80. doi:10.1016/S0888-7543(05)80302-7. PMID 1385299.

2.      Inazawa J, Itoh N, Abe T, Nagata S (November 1992). "Assignment of the human Fas antigen gene (Fas) to 10q24.1". Genomics 14 (3): 821–2. doi:10.1016/S0888-7543(05)80200-9. PMID 1385309.

3.      Wajant H (2002). "The Fas signaling pathway: more than a paradigm.". Science 296 (5573): 1635–6. doi:10.1126/science.1071553. PMID 12040174.

4.      "OrthoMaM phylogenetic marker: FAS coding sequence".

5.      Izquierdo JM, Majós N, Bonnal S, Martínez C, Castelo R, Guigó R, Bilbao D, Valcárcel J (August 2005). "Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition". Mol. Cell 19 (4): 475–84. doi:10.1016/j.molcel.2005.06.015. PMID 16109372.