• PN: B111364

COMPONENTS

45x Ab-conjugated beads (S4P4- human FGF-4 Ab-bead). PN: B111364A. One vial containing 100 µL of anti-human FGF-4 conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (human FGF-4 Biotin-dAb). PN: B111364B. One vial containing 100 µL of biotinylated anti-human FGF-4.

Lyophilized Standard Mix-Human Group 10, Panel A, 9-Plex. PN: HG1009A. One vial containing lyophilized recombinant BTC, CNTF, FGF-4, Follistatin, IL-19, KGF, PDGF-AA, PDGF-BB, and PIGF-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human FGF-4. Can be multiplexed with other analytes in Human Group 10.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 50 pg/mL

Quantitation range:

LLOQ: < 100 pg/mL

ULOQ: > 20,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 10: Negligible

Sample volume: 15 µL/test

Description:

Fibroblast growth factor 4 (FGF-4) is a protein that is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Diseases associated with FGF4 include gallbladder squamous cell carcinoma and apert syndrome.

References:

1.       Galland F, Stefanova M, Lafage M, Birnbaum D (Jul 1992). "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23". Cytogenetics and Cell Genetics 60 (2): 114–6. doi:10.1159/000133316. PMID 1611909.

2.       Feldman B, Poueymirou W, Papaioannou VE, DeChiara TM, Goldfarb M (Jan 1995). "Requirement of FGF-4 for postimplantation mouse development". Science 267 (5195): 246–9. doi:10.1126/science.7809630. PMID 7809630.

3.       Yuan H, Corbi N, Basilico C, Dailey L (Nov 1995). "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3". Genes & Development 9 (21): 2635–45. doi:10.1101/gad.9.21.2635. PMID 7590241.