- PN: B111381
45x Ab-conjugated beads (S4P10 - human GASP-1 Ab-bead). PN: B111381A. One vial containing 100 µL of anti-human GASP-1 conjugated to AimPlex Bead S4P10.
25x Biotin-detection Ab (human GASP-1 Biotin-dAb). PN: B111381B. One vial containing 100 µL of biotinylated anti-human GASP-1.
Lyophilized Standard Mix-Human Group 4 Panel B, 10-Plex. PN: HG40010. One vial containing lyophilized recombinant human GASP-1, IFNβ, ANGPT-1, Insulin, Leptin, MIF, Resistin, Survivin, TGFα, and TPO. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human GASP-1. Can be multiplexed with other analytes in Human Group 4. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 4: Negligible
Sample volume: 15 µL/test
Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that co-purify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass. Two related human proteins are called WFIKKN and WFIKKNRP (WFIKKN-related protein). Mouse GASP-1 is homologous to human WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKNRP. GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver. In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP‑2 is in the pancreas, liver, and thymus. GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro. In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide.
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