• PN: B112482

COMPONENTS

45x Ab-conjugated beads (S4P6 - Human GCP-2 Ab-bead). PN: B112482A. One vial containing 100 µL of anti-Human GCP-2conjugated to AimPlex Bead S4P6.

25x Biotin-detection Ab (Human GCP-2 Biotin-dAb). PN: B112482B. One vial containing 100 µL of biotinylated anti-Human GCP-2

Lyophilized Standard Mix-Human Group 7, Panel A, 8-Plex. PN: HG71008. One vial containing lyophilized recombinant Eotaxin-3, GCP-2, GROα, HCC-1, HCC-4, I-309, MCP-2, and MPIF-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human CXCL6/GCP-2. Can be multiplexed with other analytes in Human Group 7.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < `10 pg/mL

ULOQ: > 2,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 7: Negligible

Sample volume: 15 µL/test

Description:

Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as Granulocyte Chemotactic Protein 2 (GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2. Diseases associated with CXCL6 include periodontal disease.

References:

1.      Proost P, Wuyts A, Conings R, Lenaerts J, Billiau A, Opdenakker G, Van Damme J (1993). "Human and bovine granulocyte chemotactic protein-2: complete amino acid sequence and functional characterization as chemokines". Biochemistry 32 (38): 10170–7. doi:10.1021/bi00089a037. PMID 8399143.

2.      Wuyts A, Van Osselaer N, Haelens A, Samson I, Herdewijn P, Ben-Baruch A, Oppenheim J, Proost P, Van Damme J (1997). "Characterization of synthetic human granulocyte chemotactic protein 2: usage of chemokine receptors CXCR1 and CXCR2 and in vivo inflammatory properties". Biochemistry 36 (9): 2716–23. doi:10.1021/bi961999z. PMID 9054580.

3.      Modi W, Chen Z (1998). "Localization of the human CXC chemokine subfamily on the long arm of chromosome 4 using radiation hybrids". Genomics 47 (1): 136–9. doi:10.1006/geno.1997.5100. PMID 9465307.

4.    O'Donovan N, Galvin M, Morgan J (1999). "Physical mapping of the CXC chemokine locus on human chromosome 4". Cytogenet Cell Genet 84 (1–2): 39–42. doi:10.1159/000015209. PMID 10343098.