• PN: B111383

COMPONENTS

45x Ab-conjugated beads (S4P12 - Human GDF-15 Ab-bead). PN: B111383A. One vial containing 100 µL of anti-Human GDF-15 conjugated to AimPlex Bead S4P12.

25x Biotin-detection (GDF-15 Biotin-dAb). PN: B111383B. One vial containing 100 µL of biotinylated anti-Human GDF-15.

Lyophilized Standard Mix-Human Group 8, Panel B, 11-Plex. PN: HG82011. One vial containing lyophilized recombinant Cystatin C, FGF-1, FGF-19, Flt-3L, GDF-15, Granzyme B, NGAL, PAPP-A, SLPI, RAGE, and ST2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human GDF-15/MIC-1/PTGFB. Can be multiplexed with other analytes in Human Group 8.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 2 pg/mL

Quantitation range:

LLOQ: < 5 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 8: Negligible

Sample volume: 15 µL/test

Description:

Growth differentiation factor 15 (GDF-15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes.  GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB.  GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. Diseases associated with GDF15 include estrogen resistance and oral cavity cancer.

References:

1.      Zimmers T, Jin X, Hsiao E, McGrath S, Esquela A, Koniaris L (2005). "Growth differentiation factor-15/macrophage inhibitory cytokine-1 induction after kidney and lung injury". Shock 23 (6): 543–8. PMID 15897808.

2.      Hsiao E, Koniaris L, Zimmers-Koniaris T, Sebald S, Huynh T, Lee S (2000). "Characterization of growth-differentiation factor 15, a transforming growth factor beta superfamily member induced following liver injury". Mol Cell Biol 20 (10): 3742–3751. doi:10.1128/MCB.20.10.3742-3751.2000. PMC 85678. PMID 10779363.

3.      Ago T, Sadoshima J (2006). "GDF15, a cardioprotective TGF-beta superfamily protein". Circ Res 98 (3): 294–297. doi:10.1161/01.RES.0000207919.83894.9d. PMID 16484622.