- PN: B112488
45x Ab-conjugated beads (S4P10 - Human HCC-1 Ab-bead). PN: B112488A. One vial containing 100 µL of anti-Human HCC-1 conjugated to AimPlex Bead S4P10.
25x Biotin-detection Ab (Human HCC-1 Biotin-dAb). PN: B112488B. One vial containing 100 µL of biotinylated anti-Human HCC-1.
Lyophilized Standard Mix-Human Group 7, Panel A, 8-Plex. PN: HG71008. One vial containing lyophilized recombinant Eotaxin-3, GCP-2, GROα, HCC-1, HCC-4, I-309, MCP-2, and MPIF-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human CCL14/SCYA14/HCC-1. Can be multiplexed with other analytes in Human Group 7. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
LLOQ: < `20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 7: Negligible
Sample volume: 15 µL/test
Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family, also called Small Inducible Cytokine Subfamily A (Cys-Cys) 14 (SCYA14) and as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
1. Schulz-Knappe et al., HCC-1, a novel chemokine from human plasma. J. Exp. Med., 1996, 183: 295-299.
2. Naruseet al., A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2. Genomics, 1996, 34: 236-240.
3. CX3CL1 and CCL14 regulate extracellular matrix and adhesion molecules in the trophoblast: potential roles in human embryo implantation. (PMID: 18367676) Hannan N.J. … Salamonsen L.A.(Biol. Reprod. 2008)