• PN: B112489

COMPONENTS

45x Ab-conjugated beads (S5P5 - Human HCC-4 Ab-bead). PN: B112489A. One vial containing 100 µL of anti-Human HCC-4 conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (Human HCC-4 Biotin-dAb). PN: B112489B. One vial containing 100 µL of biotinylated anti-Human HCC-4.

Lyophilized Standard Mix-Human Group 7, Panel A, 8-Plex. PN: HG71008. One vial containing lyophilized recombinant Eotaxin-3, GCP-2, GROα, HCC-1, HCC-4, I-309, MCP-2, and MPIF-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human CCL16/SCYA16/HCC-4. Can be multiplexed with other analytes in Human Group 7.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < `20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 7: Negligible

Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Small Inducible Cytokine Subfamily A (Cys-Cys) 16 (SCYA16), Liver-expressed Chemokine (LEC), Monotactin-1 (MTN-1) and HCC-4. This chemokine is expressed by the liver, thymus, and spleen and is chemo-attractive for monocytes and lymphocytes.  Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-γ and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8. Recombinant HCC-4 has been shown to chemo-attract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.

References:

1.      Youn et al. Isolation and characterization of LMC, a novel lymphocyte and monocyte chemoattractant human CC chemokine, with myelosuppressive activity. Biochem. Biophys. Res. Commun. 247:217-222 (1998).

2.      Nomiyama et al. Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, LEC, and RANTES. J. Interferon Cytokine Res. 19:227-234 (1999).

3.      Nomiyama et al. Human CC chemokine liver-expressed chemokine/CCL16 is a functional ligand for CCR1, CCR2 and CCR5, and constitutively expressed by hepatocytes. Int Immunol. 2001 Aug;13(8):1021-9.

4.      Howard et al. LEC induces chemotaxis and adhesion by interacting with CCR1 and CCR8. Blood. 2000 Aug 1;96(3):840-5.