96 Tests PN: B111116
45x Ab-conjugated beads (S5P12 - human HGF Ab-bead). PN: B111116A. One vial containing 100 µL of anti-human HGF conjugated to AimPlex Bead S5P12.
25x Biotin-detection Ab (human HGF Biotin-dAb). PN: B111116B. One vial containing 100 µL of biotinylated anti-human HGF.
Lyophilized Standard - Human HGFx. PN: B111116S. One vial containing lyophilized recombinant HGF.
Application: Optimal antibody pair and antigen standard for assaying human HGF. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Sample volume: 15 µL/test
Hepatocyte growth factor (HGF), also known as Hepatopoietin A, HPTA and SF, is mitogenic for a variety of cell types, including endothelial and epithelial cells, melanocytes, and keratinocytes. It is identical to scatter factor, a fibroblast-derived soluble factor that promotes the dissociation of epithelial and vascular endothelial cell colonies in monolayer cultures by stimulating cell migration. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF has been implicated in a variety of cancers, including of the lungs, pancreas, thyroid, colon, and breast.
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Thomas R. Ziegler; Glenn F. Pierce; David N. Herndon (6 December 2012). Growth Factors and Wound Healing: Basic Science and Potential Clinical Applications. Springer Science & Business Media. pp. 311–. ISBN 978-1-4612-1876-0.