• PN: B113379

COMPONENTS

45x Ab-conjugated beads (S4P7-human sHVEM Ab-bead). PN: B113379A. One vial containing 100 µL of anti-human sHVEM conjugated to AimPlex Bead S4P7.

25x Biotin-detection (human sHVEM Biotin-dAb). PN: B113379B. One vial containing 100 µL of biotinylated anti-human sHVEM.

Lyophilized Standard Mix-Human Group 9 Panel B, 9-Plex. PN: HG9009B. One vial containing lyophilized recombinant human sCEACAM-1, sALCAM, sSLAMF4, sHVEM, sB7-DC, sB7-H1, sEpCAM, sBLAME and sEPOR.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human TNFRSF14/sCD270/HVEM. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 9: Negligible

Sample volume: 15 µL/test

Description:

Tumor Necrosis Factor Receptor SuperFamily member 14 (TNFRSF14), also known as Herpesvirus entry mediator (HVEM) and CD270, is a human cell surface receptor of the TNF-receptor superfamily. It is also referred to as ATAR (another TRAF-associated receptor). This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. In melanocytic cells TNFRSF14 gene expression may be regulated by MITF. Mutations in this gene have been recurrently been associated to cases of diffuse large B-cell lymphoma. Diseases associated with TNFRSF14 include herpes simplex and follicular lymphoma.

References:

1.      Montgomery RI, Warner MS, Lum BJ, Spear PG (Dec 1996). "Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family". Cell 87 (3): 427–36. doi:10.1016/S0092-8674(00)81363-X. PMID 8898196.

2.      Kwon BS, Tan KB, Ni J, Oh KO, Lee ZH, Kim KK, Kim YJ, Wang S, Gentz R, Yu GL, Harrop J, Lyn SD, Silverman C, Porter TG, Truneh A, Young PR (June 1997). "A newly identified member of the tumor necrosis factor receptor superfamily with a wide tissue distribution and involvement in lymphocyte activation". J Biol Chem 272 (22): 14272–6. doi:10.1074/jbc.272.22.14272. PMID 9162061.

3.      Ware, Carl (2008). "Chapter 25: TNF-Related Cytokines in Immunity". In Paul, William. Fundamental Immunology (Book) (6th ed.). Philadelphia: Lippincott Williams & Wilkins. pp. 776–801. ISBN 0-7817-6519-6.