• PN: B117335

COMPONENTS

45x Ab-conjugated beads (S4P2 - Human IgA Ab-bead). PN: B117335A. One vial containing   100 µL of anti-human IgA conjugated to AimPlex Bead S4P2.

25x Biotin-detection Ab (Human IgA Biotin-dAb). PN: B117335B. One vial containing 100 µL of biotinylated anti-human IgA.

Lyophilized Standard Mix - Human IgA. PN: B117335S. One vial containing lyophilized IgA.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human IgA. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Important Note: Some specimens may contain high level of IgA. Pre-diluting normal serum and plasma samples 2,000 fold with the Sample Dilution Buffer (PN: P830100) is recommended for the IgA assay. Dilution factors should be optimized according to specific experimental conditions and sample types

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 1 ng/mL

Quantitation range:

LLOQ: < 5 ng/mL

ULOQ: > 2,000 ng/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Immunoglobulin A (IgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined; between three and five grams are secreted into the intestinal lumen each day. This accumulates up to 15% of the total immunoglobulin produced in the entire body. In its secretory form (sIgA), IgA is the main immunoglobulin found in mucous secretions, including tears, saliva, sweat, colostrum and secretions from the genitourinary tract, gastrointestinal tract, prostate and respiratory epithelium. It is also found in small amounts in blood. The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes, thus sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. sIgA can also inhibit inflammatory effects of other immunoglobulins.

References:

1.      S Fagarasan; T Honjo (2003). "Intestinal IgA Synthesis: Regulation of Front-line Body Defenses". Nature Reviews Immunology. 3 (1): 63–72. doi:10.1038/nri982. PMID 12511876.

2.      P. Brandtzaeg; R. Pabst (2004). "Let's go mucosal: communication on slippery ground". Trends Immunology. 25 (11): 570–577. doi:10.1016/j.it.2004.09.005. PMID 15489184.

3.      AJ Macpherson; E Slack. (2007). "The functional interactions of commensal bacteria with intestinal secretory IgA.". Current Opinion in Gastroenterology. 23 (6): 673–678. doi:10.1097/MOG.0b013e3282f0d012. PMID 17906446.

4.       Junqueira, Luiz C.; Jose Carneiro (2003). Basic Histology. McGraw-Hill. ISBN 0-8385-0590-2.

5.      Holmgren, J; Czerkinsky, C (April 2005). "Mucosal immunity and vaccines". Nature Medicine. 11 (4s): S45–S53. doi:10.1038/nm1213. PMID 15812489.