• PN: B114560

COMPONENTS

45x Ab-conjugated beads (S5P9 - human IGFBP-2 Ab-bead). PN: B114560A. One vial containing 100 µL of anti-human IGFBP-2 conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (human IGFBP-2 Biotin-dAb). PN: B114560B. One vial containing  100 µL of biotinylated anti-human IGFBP-2.

Lyophilized Standard Mix - Human IGFBP-2. PN: B114560S. One vial containing lyophilized IGFBP-2.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human IGFBP-2. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 100 pg/mL

Quantitation range:

LLOQ: < 200 pg/mL

ULOQ: > 50,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

IGFBP2 is observed mainly in brain and liquor, showing complex patterns of gene expression during postnatal brain development. Elevated levels IGFBP2 have been observed in the serum of prostate cancer patients. IGFBP2 expression does not depend on Growth hormone. The gene maps to human chromosome 2q33-q34. It contains four exons and has a length of 32 kb. IGFBP2 has been shown to be secreted by intestinal epithelial cells and to capable of limiting the mitogenic activity of both exogenous and endogenous IGF by blocking the association of the growth factors with cell surface binding sites. IGFBP2 has been implicated also in myeloblast differentiation. It shows preferential affinity for IGF-2. Diseases associated with IGFBP2 include malignant ovarian cyst and insulin-like growth factor i.

References:

1.      Agarwal N et al Sequence analysis, expression and chromosomal localizationof a gene, isolated from a subtracted human retina cDNA library, that encodes an insulin-like growth factor binding protein (IGFBP2). Experimental Eye Research 52: 549-561 (1991);

2.      Alitalio T et al The gene encoding human low molecular weight insulin-like growth factor binding protein (IGF-BP25): regional localization to 7p12-p13 and description of a DNA polymorphism. Human Genetics 83: 335-338 (1989);

3.      Binkert C et al Structure of the human insulin-like growth factor binding protein-2 gene. Molecular Endocrinology 6: 826-836 (1992);

4.      Binkert C et al Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein IGFBP-2. EMBO Journal 8: 2497-2502 (1989)