• PN: B114575

COMPONENTS

45x Ab-conjugated beads (S5P10 - Human IGFBP-7 Ab-bead). PN: B114575A. One vial containing 100 µL of anti-Human IGFBP-7 conjugated to AimPlex Bead S5P10.

25x Biotin-detection Ab (Human IGFBP-7 Biotin-dAb). PN: B114575B. One vial containing 100 µL of biotinylated anti-Human IGFBP-7.

Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IGFBP-7. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 6: Negligible

Sample volume: 15 µL/test

Description:

Insulin-like growth factor-binding protein 7 (IGFBP-7) is a protein required for the regulation of the availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP-7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers. Diseases associated with IGFBP7 include retinal arterial macroaneurysm with supravalvular pulmonic stenosis and familial retinal arterial macroaneurysm.

References:

1.      Kato MV A secreted tumor-suppressor, mac25, with activin-binding activity. Molecular Medicine 6(2): 126-135 (2000);

2.      Oh Y et al Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7: Recombinant human mac25 protein specifically binds IGF-I and -II. Journal of Biological Chemistry 271(48): 30322-30325 (1996)

3.      Oh Y, Nagalla SR, Yamanaka Y, Kim HS, Wilson E, Rosenfeld RG (November 1996). "Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II". J. Biol. Chem. 271 (48): 30322–5. doi:10.1074/jbc.271.48.30322. PMID 8939990.

4.      Gommans WM, Tatalias NE, Sie CP, Dupuis D, Vendetti N, Smith L, Kaushal R, Maas S (October 2008). "Screening of human SNP database identifies recoding sites of A-to-I RNA editing". RNA 14 (10): 2074–85. doi:10.1261/rna.816908. PMC 2553741. PMID 18772245.

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