- PN: A111143
45x Ab-conjugated beads (S5P12 - human IL-13 Ab-bead). PN: A111143A. One vial containing 100 µL of anti-human IL-13 conjugated to AimPlex Bead S5P12.
25x Biotin-detection Ab (human IL-13 Biotin-dAb). PN: A111143B. One vial containing 100 µL of biotinylated anti-human IL-13.
Lyophilized Standard Mix-Human Group 2 Panel A, 10-Plex. PN: HG2009A. One vial containing lyophilized recombinant human G-CSF, GM-CSF, IFNα2, IL-1α, IL-3, IL-5, IL-7, IL-11, IL-12p40, and IL-13. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-13. Can be multiplexed with other analytes in Human Group 2. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 1: Negligible
Sample volume: 15 µL/test
Interleukin 13 (IL-13) is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, a mediator of allergic inflammation and disease. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL-13 induces a class of protein-degrading enzymes, known as matrix metalloproteinases (MMPs), in the airways. These enzymes are required to induce egression of effete parenchymal inflammatory cells into the airway lumen where they are then cleared. Among other factors, IL-13 induces these MMPs as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 specifically induces physiological changes in parasitized organs that are required to expel the offending organisms or their products. For example, expulsion from the gut of a variety of mouse helminths requires IL-13 secreted by Th2 cells. IL-13 induces several changes in the gut that create an environment hostile to the parasite, including enhanced contractions and glycoprotein hyper-secretion from gut epithelial cells, that ultimately lead to detachment of the organism from the gut wall and their removal.
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2. McKenzie AN, Culpepper JA, de Waal Malefyt R, Brière F, Punnonen J, Aversa G, Sato A, Dang W, Cocks BG, Menon S (April 1993). "Interleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function". Proc. Natl. Acad. Sci. U.S.A. 90 (8): 3735–9. doi:10.1073/pnas.90.8.3735. PMC 46376. PMID 8097324.
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4. Zurawski G, de Vries JE (January 1994). "Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells". Immunol. Today 15 (1): 19–26. doi:10.1016/0167-5699(94)90021-3. PMID 7907877.